岭南现代临床外科2024，Vol.24Issue(1)：42-48,7.DOI:10.3969/j.issn.1009-976X.2024.01.006
敲低核糖体发生蛋白BRIX1能够抑制三阴乳腺癌细胞的增殖、迁移和侵袭
Effects of knockdown BRIX1 on proliferation,migration and invasion of triple negative breast cancer cells
摘要
Abstract
Objective To investigate the function of BRIX1,a large subunit component of ribosome,in triple negative breast cancer cell line in vitro.Methods The expression difference of BRIX1 in normal tissue and triple negative breast cancer was analyzed by GEPIA2 database.Two SiRNAs were designed and transferred into triple negative breast cancer cell lines HS578T and MDA-MB-231 respectively to silence the expression of BRIX1,and Western Blot was used to verify the knock inefficiency of BRIX1.The proliferation of cells after BRIX1 knockdown was detected by CCK-8 and colony cloning formation assay,and the invasion and migration ability of cells after BRIX1 knockdown was detected by Transwell assay.MDA-MB-231 cells were divided into three groups,which were transferred into siNC,siBRIX1#1 and siBRIX1#2 respectively,and total RNA of the cells was extracted for RNA-Seq detection,and the difference of gene expression was analyzed.Results The results of GEPIA2 database showed that BRIX1 was highly expressed in triple negative breast cancer(P<0.05),and the number of clones was decreased in HS578T and MDA-MB-231 cells(P<0.05).The OD value of cck8 was decreased,which proved that the cell proliferation activity decreased after knocking down BRIX1.TranswellTM experiment showed that the invasion and migration ability of breast cancer was weakened after knocking down BRIX1.Biological process(BP),molecular function(MF),and cell composition(CC)of RNA-seq data were enriched by GO,and the top10 signaling pathways with high correlation were finally revealed.The results showed that after knockdown BRIX1,ERBB-ERK and WNT signaling pathways were activated,while P53 signaling pathways were inhibited.Conclusion Down-regulated BRIX1 expression could significantly inhibited the proliferation,migration and invasion of triple negative breast cancer cells,it suggesting that BRIX1 gene could be a potential molecular target for triple negative breast cancer therapy.关键词
三阴乳腺癌BRIX1/细胞增殖迁移侵袭Key words
triple negative breast cancer/BRIX1/proliferation/migration and invasion分类
医药卫生引用本文复制引用
董一鸣,石川平,廖建友..敲低核糖体发生蛋白BRIX1能够抑制三阴乳腺癌细胞的增殖、迁移和侵袭[J].岭南现代临床外科,2024,24(1):42-48,7.基金项目
国家自然科学基金面上项目(82072924) （82072924）
