岭南现代临床外科2024，Vol.24Issue(1)：54-58,5.DOI:10.3969/j.issn.1009-976X.2024.01.008
HIST1H4F基因甲基化在三阴性乳腺癌中的预后价值
Prognostic value of HIST1H4F gene methylation in triple-negative breast cancer
摘要
Abstract
Objective To investigate prognostic value of HIST1H4F gene methylation in triple-negative breast cancer.Methods Thirty-one fresh breast cancer tissue samples and normal breast tissue samples were collected.After DNA extraction,bisulfite was used for methylation transformation.Fluorescence quantitative PCR was used to detect the methylation of HIST1H4F gene,and the difference of HIST1H4F gene methylation in cancer tissue samples and normal breast tissue was analyzed.The relationship between methylation of HIST1H4F gene and prognosis of triple-negative breast cancer was analyzed.At the same time,paraffin specimens of 37 cases of triple-negative breast cancer were collected,DNA was extracted and processed by the same method,and HIST1H4F gene methylation was detected to analyze the relation-ship between HIST1H4F gene methylation and prognosis of triple-negative breast cancer.Results The HIST1H4F methylation positive rates were 74.2%(23/31)in both normal and cancer tissues,and there was no significant difference between the two groups(χ2=0.000,P=1.000).the HIST1H4F methylation positive rate in triple-negative breast cancer was higher than that in non-triple-negative breast cancer(93.8%vs.53.3%,P=0.015),HIST1H4F methylation-positive group had higher HIST1H4F grade Ⅲand ER negative ratio than HIST1H4F methylation-negative group(60.9%vs.12.5%,P=0.037;69.6%vs.25.0%,P=0.043).For HIST1H4F methylation in paraffin tissue specimens of triple-negative breast cancer and found a HIST1H4F methylation positive rate of 27.0%,there was no significant difference between HIST1H4F methylation positive and negative groups in DFS(50.0%vs.66.7%,χ2=1.141,P=0.285)and OS(90.0%vs.96.3%,χ2=1.635,P=0.201).Conclusion The positive rate of HIST1H4F gene methyl-ation in triple negative breast cancer is high in fresh tissue samples,and histological grade Ⅲ and ER negative ratios were high in HIST1H4F gene methylation-positive breast cancer.While HIST1H4F methyl-ation positive rate was low in paraffin tissue samples of triple-negative breast cancer.Whether HIST1H4F methylation is associated with triple-negative breast cancer prognosis remains to be determined and further studies are needed.关键词
乳腺癌/HIST1H4F/甲基化Key words
breast cancer/HIST1H4F/methylation分类
医药卫生引用本文复制引用
严珊珊,雷雯,李帅杰,王永南..HIST1H4F基因甲基化在三阴性乳腺癌中的预后价值[J].岭南现代临床外科,2024,24(1):54-58,5.基金项目
广东省医学科研基金(A2021063) （A2021063）
