兰州大学学报（医学版）2024，Vol.50Issue(3)：14-18,5.DOI:10.13885/j.issn.1000-2812.2024.03.003
一种新型二氧化硅多孔材料固定化酶光纤葡萄糖传感器的研究
A novel glucose optical fiber biosensor with enzyme immobilized by SiO2 porous material
摘要
Abstract
Objective To develop an optical fiber sensor with a composite sensitive film containing aerobic sensitive indicator Ru(bpy)3 Cl2 and immobilized glucose oxidase(GOD)for glucose detection.Methods In this work,an enzyme-based optical fiber fluorescence biosensor was prepared by using the SiO2 nanoparticles immobilized with GOD(SiO2-GOD),where the fluorescence indicator was Ru(bpy)3 Cl2.The pull-up method was used to deposit a sensitive film on the face of an optical fiber to produce an optical fiber sensing probe,and then optical fiber sensor was constructed to detect the different concentrations of the glucose solution.Results The optical fiber sensor detected glucose solution concentration in the range of 50-900 mg/dL(2.78～50.00 mmol/L),which could be used for the real-time monitoring of the glucose concentration changes.The response time of the sensor was short,and the testing standard curve had good linearity.Conclusion The fiber optic glucose sensor has the advantages of fast response,high sensitivity and wide detection range for glucose.In the future,it can be used to detect glucose concentration in human blood.关键词
葡萄糖检测/二氧化硅多孔材料固定化酶/荧光信号/光纤传感器Key words
glucose detection/immobilization of enzymes using porous silica materials/fluorescence signal/optical fiber sensor分类
化学化工引用本文复制引用
姚润麒,张鹏飞,高泽林,姜兴东,孟文勃,丁莉芸..一种新型二氧化硅多孔材料固定化酶光纤葡萄糖传感器的研究[J].兰州大学学报（医学版）,2024,50(3):14-18,5.基金项目
国家自然科学基金面上基金资助项目(51878524) （51878524）
湖北省自然科学基金杰出青年基金资助项目(2021CFA041) （2021CFA041）
