饲料研究2024，Vol.47Issue(7)：88-93,6.DOI:10.13557/j.cnki.issn1002-2813.2024.07.016
沙枣叶水提取物的体外抗菌活性机制研究
In vitro antibacterial activity mechanism of Elaeagnus angustifolia L.leaves water extract
摘要
Abstract
The purpose of this experiment was to determine the antibacterial activity of Elaeagnus angustifolia L.leaves water extract against pathogenic bacteria easily causing animal infection in vitro,and to reveal the antibacterial mechanism of Elaeagnus angustifolia L.leaves water extract.The antibacterial spectrum of Elaeagnus angustifolia L.leaves water extract was screened by a 96-well plate microdilution method.The growth curve of pathogenic bacteria was drawn,the activity of alkaline phosphatase and protein mass concentration were determined,and the inhibitory mechanism of Elaeagnus angustifolia L.leaves water extract against Staphylococcus aureus was comprehensively analyzed by transmission electron microscope.The results showed that the water extract of Elaeagnus angustifolia L.leaves had different inhibitory effects on Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Pseudomonas aeruginosa,Escherichia coli,Enterobacter cloacae,and Candida albicans,which could destroy the permeability and integrity of cell wall and membrane of pathogenic bacteria and inhibit their growth and metabolism.Studies has shown that the Elaeagnus angustifolia L.leaves water extract can inhibit the growth and metabolism of common Staphylococcus aureus and other pathogenic bacteria,and has a strong antibacterial function.关键词
沙枣叶/饲料添加剂/金黄色葡萄球菌/抑菌机制Key words
Elaeagnus angustifolia L.leaves/feed additives/Staphylococcus aureus/antibacterial mechanism分类
农业科技引用本文复制引用
唐莎莎,宫海燕,于浩楠,孙芸..沙枣叶水提取物的体外抗菌活性机制研究[J].饲料研究,2024,47(7):88-93,6.基金项目
国家自然科学基金项目(项目编号:81860772) （项目编号:81860772）
