饲料研究2024，Vol.47Issue(7)：94-99,6.DOI:10.13557/j.cnki.issn1002-2813.2024.07.017
Box-Behnken响应面优化超声波辅助双水相提取枇杷叶多糖及其抗氧化活性研究
Ultrasonic-assisted aqueous two-phase extraction of polysaccharide from loquat leaves by Box-Behnken response surface optimization and its antioxidant activity
摘要
Abstract
Box-Behnken design response surface methodology was used to optimize the ultrasonic-assisted aqueous two-phase extraction of loquat leaves polysaccharide.The effects of ultrasonic time,ultrasonic power,ultrasonic temperature,and liquid-material ratio on the extraction rate of loquat leaves polysaccharide and the antioxidant activity of loquat leaves polysaccharide were studied.The results showed that the optimum conditions for extracting polysaccharide from loquat leaves by ultrasonic-assisted aqueous two-phase method were ultrasonic time 60 min,ultrasonic power 180 W,ultrasonic temperature 60 ℃,liquid-material ratio 70 mL/g,and the yield of polysaccharide from loquat leaves reached 4.222％.The antioxidant activity test showed that the DPPH radical scavenging rate was 73.90％and the hydroxyl radical scavenging rate was 58.45％when the concentration of loquat leaves polysaccharide was 1.2 g/L.The study indicates that the extraction technology of loquat leaves polysaccharide is environmentally friendly and feasible,and loquat leaves polysaccharide have good antioxidant activity.关键词
枇杷叶/多糖/超声波/双水相/抗氧化Key words
loquat leaves/polysaccharide/ultrasonic wave/aqueous two-phase/antioxidant分类
农业科技引用本文复制引用
玉澜,肖云,陈燕萌,郭松..Box-Behnken响应面优化超声波辅助双水相提取枇杷叶多糖及其抗氧化活性研究[J].饲料研究,2024,47(7):94-99,6.基金项目
广西高校中青年教师科研基础能力提升项目(项目编号:2021KY0862) （项目编号:2021KY0862）
广西科技师范学院壮瑶药品质生物学重点实验室经费(项目编号:GXKSKYPT2021007) （项目编号:GXKSKYPT2021007）
广西科技基地和人才专项(项目编号:2021AC19423) （项目编号:2021AC19423）
广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目 （中心）
