饲料研究2024，Vol.47Issue(8)：88-91,4.DOI:10.13557/j.cnki.issn1002-2813.2024.08.016
牛大力多糖的荧光标记、鉴定及其体内分布
Fluorescence labeling,identification,and in vivo distribution of Millettia speciosa polysaccharide
摘要
Abstract
The study was to explore the absorption and distribution of Millettia speciosa polysaccharide in mice.The Millettia speciosa polysaccharide Rhodamine B fluorescent marker(MSP-Rh B)was obtained by labeling Millettia speciosa polysaccharide with Rhodamine B.After the labels were identified by fluorescence microscope and infrared spectrum scanning,MSP-Rh B was given to mice by intragastric administration.Samples were collected at 1,3,6,12,24 h,respectively,and the distribution of MSP-Rh B in vivo and in vitro was observed by small animal in vivo imaging system.The results showed that fluorescence labeling of Millettia speciosa polysaccharide was successful.After intragastric administration,MSP-Rh B was mainly distributed in gastrointestinal tract,liver and kidney.MSP-Rh B reached each segment of the small intestine at 1 h,reached its maximum at about 3 h,and stayed in the small intestine for 24 h.Studies have shown that MSP-Rh B is absorbed through the small intestine,enters the systemic circulation first,and then distributes to the liver and kidney,and the fluorescence signal gradually decreases with time.关键词
牛大力多糖/荧光标记/口服吸收/体内分布Key words
Millettia speciosa polysaccharide/fluorescence labeling/oral absorption/in vivo distribution分类
农业科技引用本文复制引用
多水银,张兴波,吴玉辉,李静涛,杨雨辉..牛大力多糖的荧光标记、鉴定及其体内分布[J].饲料研究,2024,47(8):88-91,4.基金项目
国家自然科学基金项目(项目编号:32260900) （项目编号:32260900）
海南省自然科学基金高层次人才项目(项目编号:321RC481) （项目编号:321RC481）
