饲料研究2024，Vol.47Issue(8)：132-137,6.DOI:10.13557/j.cnki.issn1002-2813.2024.08.024
HPLC法同时测定三味拳参口服液中苦参碱和氧化苦参碱的含量
Simultaneous determination of matrine and oxymatrine in Sanwei Quanshen Koufuye by HPLC
摘要
Abstract
The study was to establish a method for the simultaneous determination of matrine and oxymatrine in Sanwei Quanshen Koufuye by HPLC.The test compares the detection effects of three amino-chromatographic columns(Agilent ZORBAX NH2,SHISEIDO CAPCELL PAK NH2,Waters SPHERSORB NH2).For separation effect,Agilent ZORBAX NH2 is preferred,but its running time is long.In order to save time and flow equivalent reagents,it was recommended to use SHISEIDO CAPCELL PAK NH2 column first and subselect Waters SPHERSORB NH2 column.After the absolute ethanol extraction,the amino chromatography column(4.6 mm × 250 mm,5 pm)was used as the separation column,column temperature was 35 ℃,and detection wavelength was 220 nm.Diode array detector was used for analysis,and external standard method was used for quantitative determination.The results showed that matrine and oxymatrine showed a good linear relationship ranging between 30 and 300 mg/L(R＞0.999 0),and the average sample recovery rate was 97.64％with RSD 1.63％.The system adaptation test and the chromatography column tolerance was good,which fully meet the detection requirements.The study indicates that the method is easy to operate,with good repeatability,stability and accuracy,and can be used for the detection of matrine and oxymatrine contents in Sanwei Quanshen Koufuye.关键词
苦参碱和氧化苦参碱/高效液相色谱法/二极管阵列检测器/三味拳参口服液Key words
matrine and oxymatrine/HPLC/diode array detector/Sanwei Quanshen Koufuye分类
农业科技引用本文复制引用
魏秀丽,张传津,李有志,杨志昆,张志民,冯涛,王尚明..HPLC法同时测定三味拳参口服液中苦参碱和氧化苦参碱的含量[J].饲料研究,2024,47(8):132-137,6.基金项目
山东省重点研发计划(项目编号:2022CXPT022) （项目编号:2022CXPT022）
泰山产业创新领军人才工程专项(项目编号:TSCX202211023) （项目编号:TSCX202211023）
2020年度兽药国家标准制修订任务(项目编号:ZY-2020-12) （项目编号:ZY-2020-12）
