丁酸梭菌对钝吻黄盖鲽幼鱼生长性能、肠道结构和肠道菌群的影响OA

In order to study the effects of dietary Clostridium butyricum supplementation on the growth and intestinal health of juvenile Pseudopleuronectes yokohamae,a total of 180 juveniles of P.yokohamae with the average body length of(10.28±0.08)cm and average body weight of(13.72±0.90)g were randomly divided into the experimental group(dietary C.butyricum liquid supplemented treatment)and the control group,and a 42-day culture experiment was carried out.Body size measurement,intestinal tissue section observation and high-throughput sequencing were used to study the effects of C.butyricum solution on the growth performance,intestinal structure and intestinal flora of P.yokohamae.The results showed that the weight gain rate(P<0.01),specific growth rate(P<0.05)and fullness(P<0.05)of juveniles in the experimental group were significantly increased,and the feed coefficient ratio(P<0.05)was significantly decreased,when compared to the control group.The length(P<0.01)and width(P<0.05)of villi were also significantly increased in the intestinal tissue of experi-mental group.The results of Illumina MiSeq sequencing showed that the experimental group and the control group showed different degrees of differences at the phylum and genus levels.C.butyricum can increase the number of potentially beneficial bacteria in the intestinal tract of P.yokohamae,and promote the function of intestinal break-down and metabolism of feed.The results showed that the addition of C.butyricum to the feed could improve the growth performance,improve the intestinal structure,regulate the proportion of intestinal flora,and promote intestinal health of juveniles P.yokohamae.