实用医学杂志2024，Vol.40Issue(10)：1344-1350,7.DOI:10.3969/j.issn.1006-5725.2024.10.003
S100A7A在胃癌中的表达及对增殖转移的影响
Expression of S100A7A in gastric cancer and its effect on proliferation and metastasis
摘要
Abstract
Objective The objective of this study is to examine the expression level of the S100A7A protein in both gastric cancer tissues and cells,as well as to evaluate its impact on the malignant phenotype of gastric cancer(GC)cells.Methods Immunohistochemical assay was used to detect the expression characteristics of S100A7A in 21 gastric cancer tissues and their corresponding paracancerous tissues,as well as to investigate its correlation with gastric cancer clinicopathological factors.Gastric cancer cells were genetically modified to overex-press S100A7A through plasmid transfection.Subsequently,the impact of S100A7A on the proliferation,migra-tion,and invasion capacities of gastric cancer cells was assessed using cell proliferation assays(EdU assay and plate cloning assay)as well as cell migration and invasion assays(Transwell assay and scratch assay).Results The expression of S100A7A protein was higher in GC tissues than in paracancerous tissues;Overexpression of S100A7A may increase gastric cancer cell proliferation,migration,and invasion.Conclusion S100A7A is a possible oncogene in GC and is predicted to serve as a new diagnostic and therapeutic target for the disease.关键词
胃癌/S100A7A/增殖/迁移/侵袭Key words
gastric cancer/S100A7A/proliferation/migration/invasion分类
医药卫生引用本文复制引用
肖无双,王继德,洪林杰,余针,杨萍,张杰铭,彭思扬,魏向阳,陈奕东,刘思德..S100A7A在胃癌中的表达及对增殖转移的影响[J].实用医学杂志,2024,40(10):1344-1350,7.基金项目
国家自然科学基金项目(编号:81974448,82273354) （编号:81974448,82273354）
广东省自然科学基金项目(编号:2022A1515012464) （编号:2022A1515012464）
