实用医学杂志2024，Vol.40Issue(10)：1357-1363,7.DOI:10.3969/j.issn.1006-5725.2024.10.005
原花青素对大鼠背根神经节神经元突起生长的影响
Proanthocyanidins promotes neurite outgrowth of dorsal root ganglion neurons in rat
摘要
Abstract
Objective To investigate the effect of proanthocyanidins(PC)on the neurite outgrowth of rat dorsal root ganglion(DRG)neurons.Methods In vitro,primary rat DRG neurons were cultured wtih a series of concenteation of PC to assess the effect of PC on the number and length of neurites as well as the morphology of growth cone.In vivo,the expression of growth associated protein 43(GAP43)in the early stage of injury was detected using the sciatic nerve crush model.Finally,the impact of PC on nerve growth factor(NGF)expression in DRG neurons was evaluated in vitro using immunofluorescence and ELISA.Results PC significantly increased the number and length of neurites and the number of pseudopodium in growth cones of DRG neurons.PC also promoted the expres-sion of GAP43 in the early stage of sciatic nerve injury in rats and enhanced the expression of NGF in DRG neurons.Conclusion PC may promote the neurite outgrowth by increasing the expression of NGF in DRG neurons.关键词
原花青素/神经元/突起生长/神经生长因子Key words
proanthocyanidins/neurons/neurite outgrowth/nerve growth factor分类
医药卫生引用本文复制引用
陈颖秀,郭映琪,张惠媚,张琪,文锦坤,罗利,李莉霞..原花青素对大鼠背根神经节神经元突起生长的影响[J].实用医学杂志,2024,40(10):1357-1363,7.基金项目
广东省医学科研基金项目(编号:A2021466) （编号:A2021466）
广东省普通高校青年创新人才类项目(编号:2021KQNCX034) （编号:2021KQNCX034）
广州市科技计划项目(编号:2023A04J1138) （编号:2023A04J1138）
