实用医学杂志2024，Vol.40Issue(10)：1382-1388,7.DOI:10.3969/j.issn.1006-5725.2024.10.009
神经胶质瘤组织中miR-203a-3p、PHOX2B表达水平与临床特征及预后的关系
Correlations of miR-203a-3p and PHOX2B expression in glioma tissues with clinical features and prognosis
摘要
Abstract
Objective To investigate the correlations microRNA-203a-3p(miR-203a-3p)and paired allographic box protein 2B(PHOX2B)expression levels in glioma tissues with clinical features and prognosis.Methods Ninety-six glioma patients admitted to our hospital from June 2017 to April 2020 were included in the study.The resected glioma tissues and corresponding paracancerous tissues from the patients were collected intraop-eratively.qRT-PCR and immunohistochemistry were conducted to detect the expression of miR-203a-3p and PHOX2B mRNA in glioma tissues and paracancerous groups.Pearson's method was used to analyze the correlation between miR-203a-3p and PHOX2B mRNA.Kaplan-Meier survival curves were used to analyze the correlations of miR-203a-3p and PHOX2B mRNA with the prognosis of glioma patients.COX regression was used to analyze the risk factors affecting the prognosis of glioma patients.Results The miR-203a-3p level and positive expression rate of glioma tissues were significantly lower than that of paracancerous tissues(P<0.05),and the PHOX2B mRNA level and positive expression rate were significantly higher than that of paracancerous tissues(P<0.05).By Pearson correlation analysis,the miR-203a-3p and PHOX2B mRNA levels of glioma patients were negatively correlated(P<0.05).miR-203a-3p and PHOX2B were associated with KPS score,tumor tissue necrosis,and WHO grading(P<0.05).Kaplan-Meier curve analysis yielded the results that the survival rate of the miR-203a-3p highly expressed group was significantly higher than that of the lowly expressed group(P<0.05),and the survival rate of the PHOX2B mRNA highly expressed group was significantly lower than that of the lowly expressed group(P<0.05).COX regression analysis showed that miR-203a-3p,PHOX2B mRNA,tumor tissue necrosis,and WHO grading were the risk factors affecting the prognosis of glioma patients(P<0.05).Conclusion The expression level of miR-203a-3p is obviously reduced and the expression level of PHOX2B mRNA is obviously increased in glioma,both showing close correlation with clinical characteristics and prognosis.关键词
微小RNA-203a-3p/配对同源异型盒蛋白2B/神经胶质瘤/临床特征/预后Key words
micro RNA-203a-3p/paired-like homebox 2B/glioma/clinical characteristics/prog-nosis分类
医药卫生引用本文复制引用
张淇,胡嘉,李辉,王锦,李忠德,胡志源..神经胶质瘤组织中miR-203a-3p、PHOX2B表达水平与临床特征及预后的关系[J].实用医学杂志,2024,40(10):1382-1388,7.基金项目
甘肃省自然科学基金项目(编号:22JR5RG571) （编号:22JR5RG571）
