上海农业学报2024，Vol.40Issue(2)：11-19,9.DOI:10.15955/j.issn1000-3924.2024.02.03
基于BSA-seq技术对西瓜抗枯萎病生理小种1的基因定位
Mapping of watermelon(Citrullus lanatus)Fusarium wilt-resistance gene Fon-1 based on BSA-Sequencing technology
摘要
Abstract
Watermelon Fusarium wilt,a worldwide soil borne fungal disease,is caused by Fusarium oxysporum f.sp.niveum and can dramatically reduce the watermelon yield.In this study,the resistant watermelon germplasm"V13-9-3"and the susceptible germplasm"W16-1"were used as parents to construct F1 and F2 populations.The F1 population showed resistance to Fusarium wilt,while the F2 population had a 3∶1 segregation ratio of resistance and susceptibility.It demonstrated that the resistance against F.oxysporum f.sp.niveum race 1 was controlled by a single dominant gene.Fon-1 was found to be localized to 2.20 Mb and 2.32 Mb on chromosome 1 by single nucleotide polymorphism(SNP)and insertion deletion(InDel)index methods.After accounting for overlapping regions,the target region narrowed down to 2.20 Mb.Reverse-transcription quantitative polymerase chain reaction analysis further confirmed the relative expression of genes,whereby the expression of 7 genes in"V13-9-3"was significantly higher than that in"W16-1"at 5 dpi or 10 dpi.This study proposed a novel combinatorial strategy by combining the SNP and InDel index analyses.This approach allowed rapid mapping of the genes responsible for specific traits and provides a robust base for cloning the Fon-1 gene.关键词
西瓜/枯萎病/抗枯萎病基因/基因定位/SNP指数/InDel指数Key words
Watermelon/Fusarium oxysporum/Fusarium wilt-resistance gene/Gene mapping/SNP-index/InDel-index分类
农业科技引用本文复制引用
李超汉,朱丽华,尤佳琪,杨红娟,李青竹,宋荣浩,顾卫红..基于BSA-seq技术对西瓜抗枯萎病生理小种1的基因定位[J].上海农业学报,2024,40(2):11-19,9.基金项目
上海市2020年度"科技创新行动计划"农业科技领域项目(20392000100) （20392000100）
上海市科技兴农项目(2021-02-08-00-12-F00754) （2021-02-08-00-12-F00754）
上海市农业科学院"卓越团队"建设计划[沪农科卓(2022)020] （2022）
