上海农业学报2024，Vol.40Issue(2)：66-70,5.DOI:10.15955/j.issn1000-3924.2024.02.11
原花青素添加对鸡精液冷冻效果的影响
Effects of procyanidin addition on rooster semen freezing
摘要
Abstract
In order to explore the application effects of antioxidant procyanidin(PC)in rooster semen freezing,different concentrations of PC(10 µg/mL,20 μg/mL,30 µg/mL,40 µg/mL,60 µg/mL)were added to 10％yolk supernatant dilution,the quality indexes and antioxidant indexes of frozen sperm were detected.The results showed that compared to the control,there was no significant difference in the quality indexes(survival rate,viability,membrane integrity,acrosome integrity,and mitochondrial activity)of frozen rooster sperm when 10-30 μg/mL PC was added to the yolk supernatant dilution.When adding PC with 40-60 μg/mL,all quality indexes of frozen chicken sperm significantly decreased(P＜0.05).In terms of antioxidant indexes,the addition of PC could improve the antioxidant capacity of frozen rooster sperm,among which the antioxidant capacity of frozen chicken sperm with 30 μg/mL PC addition was the highest.The MDA content and ROS content of frozen rooster sperm were the lowest,at 5.48 nmol/L and 342.00 U/mL,respectively;SOD activity and GSH-PX activity were the highest,at 137 U/mL and 90.85 U/L,respectively,with significant differences compared to other groups.When the concentration of PC added was 40-60 µg/mL,the antioxidant capacity of frozen rooster sperm significantly decreased,showing toxic side effects on sperm.It could be seen that adding 10-30 μg/mL PC during the freezing process of rooster semen may not significantly improve the quality of frozen rooster sperm,but it can significantly enhance its antioxidant capacity.关键词
鸡/精液冷冻/原花青素Key words
Chicken/Semen freezing/Proanthocyanidins分类
农业科技引用本文复制引用
何孟纤,戴建军,孙玲伟,徐皆欢,吴彩凤,张树山,滑志民,张德福..原花青素添加对鸡精液冷冻效果的影响[J].上海农业学报,2024,40(2):66-70,5.基金项目
国家重点研发计划(2021YFD1200301) （2021YFD1200301）
上海市农业科学院助跑计划人才项目(ZP22171) （ZP22171）
