实用医学杂志2024，Vol.40Issue(10)：1445-1449,5.DOI:10.3969/j.issn.1006-5725.2024.10.020
血细胞处理仪制备洗涤混合血小板及对生物活性的影响
Preparation of washed mixed platelets by blood cell processing apparatus and its effect on biological activity
摘要
Abstract
Objective To establish a new program of blood cell processing apparatus(NGL-BBS)for the preparation of washed mixed plateletsand to study the effect on biological activity of platelets compared with tradi-tional manual method.Methods Mixed concentrated platelets were separated and prepared from whole blood by white membrane method.Blood cell processing apparatus with new program set(experimental group)and manual method(control group)was used for thepreparation of washed mixed platelets.The expression rate of CD62P and CD63 in the two groups of washed mixed platelets was compared by flow-cytometry.Thrombus elastography(TEG)was used to measure and compare the MA value between the two groups.Results The expression rate of CD62P and CD63 in the experimental group was lower than that in the control group(t = 4.11,P<0.01;t = 10.78,P<0.01).TheTEG MA valueof the experimental group was higher than that of the control group(t = 6.67,P<0.01).Conclusion The present study demonstrates that the use of NGL-BBS for the preparation of washed mixed plate-lets has a lesser impact on biological activity compared to manual preparation methods.关键词
血小板/生物活性/血栓弹力图/MA值Key words
platelet/biological activity/thromboelastography/MA value分类
医药卫生引用本文复制引用
陈小光,陈茵,谭爱玲,梁明月,黄小敏,杨子曜,何博..血细胞处理仪制备洗涤混合血小板及对生物活性的影响[J].实用医学杂志,2024,40(10):1445-1449,5.基金项目
广东省自然科学基金项目(2023A1515010924) （2023A1515010924）
广东省医学科研基金项目(编号:B2023319) （编号:B2023319）
广州市医学重点学科(2021-2023年)项目 （2021-2023年）
