上海农业学报2024，Vol.40Issue(2)：94-101,8.DOI:10.15955/j.issn1000-3924.2024.02.16
小棚养殖凡纳滨对虾"滴星病"的研究
Study on"Dixing disease"of Litopenaeus vannamei cultured in small shed
摘要
Abstract
To explore the pathogenesis of Litopenaeus vannamei,a dominant strain MRY0520 was isolated from the hepatopancreas of dying diseased shrimp.Meanwhile,the strain MRY0520 was observed morphologically,identified physiologically and biochemically,and analyzed by molecular biology.The results showed that the strain MRY0520 was identified as Photobacterium damselae subsp.damselae,and the artificially infected L.vannamei could cause healthy shrimp exhibit the same symptoms as natural diseased shrimp.The results of artificial challenge showed that the LD50 of the bacteria to L.vannamei in 72 h was 2.15 x 105 CFU/g,the drug sensitivity test showed that strain MRY0520 was sensitive to neomycin(30 μg/disc),doxycycline(30 μg/disc)and other drugs.The bacteria was pathogenic to L.vannamei,and could cause obvious pathological changes in the hepatopancreas of the shrimp,even leading to death.关键词
凡纳滨对虾/美人鱼发光杆菌美人鱼亚种/分离鉴定/药敏试验Key words
Litopenaeus vannamei/Photobacterium damselae subsp.damselae/Isolation and identification/Drug sensitivity test分类
农业科技引用本文复制引用
于志君,王李宝,黎慧,史文军,赵然,万夕和..小棚养殖凡纳滨对虾"滴星病"的研究[J].上海农业学报,2024,40(2):94-101,8.基金项目
江苏省农业科技自主创新资金项目[CX(21)3160] （21）
江苏现代农业产业技术体系[JATS(2022)419、JATS(2022)164] （2022）
江苏省农业重大新品种创制项目(PZCZ201747) （PZCZ201747）
