生态环境学报2024，Vol.33Issue(4)：531-538,8.DOI:10.16258/j.cnki.1674-5906.2024.04.004
青钱柳无性系对寒驯化的生理响应及其抗寒性初步评价
Physiological Response of Cyclocarya paliurus Clones to Cold Acclimation and Preliminary Evaluation of Their Cold Resistance
摘要
Abstract
As a multi-functional tree species,Cyclocarya paliurus is mainly distributed in the subtropical regions of China.To screen out clones or varieties with stronger cold resistance,we explored the physiological response of C.paliurus clones to cold acclimation and their relationship with cold resistance to provide theoretical references for directional cultivation and cold resistance identification of C.paliurus.Using current-year branches sampled from 19 clones and the liping provenance(CK-LP)of 3-year-old C.paliurus,we measured the mass fraction of water,soluble protein,soluble sugar,starch,lignin,and hemicellulose after cold acclimation,identified the freezing injury of the plants after winter for each clone,and calculated their freezing injury indices.Correlations between physiological indicators and freezing injury indices were analyzed,and a preliminary evaluation of cold resistance was performed for the 19 clones.Our results revealed significant differences in the mass fractions of water,soluble proteins,soluble sugars,starch,lignin,and hemicellulose among the current-year branches of the tested clones.Based on the freezing injury index,cluster analysis divided the tested C paliurus clones into four categories:one superior cold resistant clone(CP3),10 good cold resistant clones(LF1,Y1,LC4,L1,CR4,J1,LC2,CR5,CR2,and BA1),four moderate cold resistant genotypes(three clones(Q4,LS3,CP6),one provenance(CK-LP),and five weak cold resistant clones(LS2,S4,CR6,LC1,and LS5).The correlation analysis indicated that the water mass fraction in the branches was positively correlated with the freezing injury index,whereas the mass fractions of soluble proteins,starch,lignin,and hemicellulose were negatively correlated with the freezing injury index.Overall,after cold acclimation,the water and soluble protein mass fractions in the current-year branches could be used as the main indicators for the early selection of clones with strong cold resistance,whereas the mass fractions of starch,lignin,and hemicellulose could be used as reference indicators.Among the tested clones of C.paliurus,clone CP3,with superior cold resistance,can be used as the preferred clone for breeding,whereas the other ten clones with good cold resistance are potential breeding materials in the future.关键词
青钱柳/寒驯化/生理响应/冻害指数/抗寒性评价/田间鉴定法Key words
Cyclocarya paliurus/cold acclimation/physiological response/freezing injury index/cold resistance evaluation/field identification method分类
农业科技引用本文复制引用
谷月营,张赞培,毛乾兴,方升佐..青钱柳无性系对寒驯化的生理响应及其抗寒性初步评价[J].生态环境学报,2024,33(4):531-538,8.基金项目
国家自然科学基金项目(32071750) （32071750）
江苏省农业重点研发项目(BE2019388) （BE2019388）
