上海农业学报2024，Vol.40Issue(2)：126-138,13.DOI:10.15955/j.issn1000-3924.2024.02.20
适配体筛选中单链DNA获取方法研究进展
Research progress on single stranded DNA acquisition methods in adapter screening
摘要
Abstract
Adaptor technology has developed rapidly in the past 20 years and has received high attention in fields such as food safety,environmental monitoring,forensic identification,and biomedicine.At present,a large number of studies have screened single stranded deoxyribonucleic acid(ssDNA)aptamers for small molecules,proteins,viruses,bacteria,cells,etc.However,ssDNA adapter screening is still a time-consuming and laborious task,usually requiring about 10 or more rounds of ssDNA-dsDNA(double stranded DNA)-ssDNA repeat steps until the sequence with strong affinity becomes the main sequence.After sequencing,one or several sequences with the strongest affinity are selected from them.Obviously,obtaining ssDNA during this process is a crucial step in adapter screening and also a rate limiting step.This article provides a systematic review of methods for obtaining ssDNA in adapter screening research.It is hoped that more in-depth research can be conducted on the acquisition of ssDNA secondary libraries in adapter screening to improve screening efficiency.关键词
适配体/筛选/单链DNA/双链DNA/聚合酶链式反应Key words
Aptamer/Screen/Single-stranded deoxyribonucleic acid(ssDNA)/Double-stranded deoxyribonucleic acid(dsDNA)/Polymerase chain reaction(PCR)分类
生物科学引用本文复制引用
白亚龙,廖小艳,普春敏,邵毅..适配体筛选中单链DNA获取方法研究进展[J].上海农业学报,2024,40(2):126-138,13.基金项目
上海市"科技创新行动计划"农业领域项目(21N31901100) （21N31901100）
上海市科技兴农项目(2022-02-08-00-12-F01089) （2022-02-08-00-12-F01089）
上海市农业科学院2022年度农业科技创新支撑领域专项-应用基础研究项目[农科应基2022(09)] （09）
