生态环境学报2024，Vol.33Issue(4)：633-644,12.DOI:10.16258/j.cnki.1674-5906.2024.04.014
天鹅湖沉积物中解磷菌的解磷能力及其对硬毛藻生长的影响
Phosphate Solubilizing Ability of Phosphate-Solubilizing Bacteria in Sediments and Its Effects on the Growth of Chaetomorpha sp.in Swan Lagoon
摘要
Abstract
Sediment is a vital nutrient source sustaining harmful algal blooms,and phosphate-solubilizing bacteria(PSB)are essential for promoting the cycle of phosphorus(P)in water.Therefore,it is of great significance to study the relationships between PSB and sediment P release during outbreak of an algal bloom.Two strains of organic phosphate-solubilizing bacteria(OPB)(Geobacillus stearothermophilus and Bacillus pumilus)isolated from Swan Lagoon and sediments collected from the algal-bloom area were employed as an indoor simulation to examine the effects of PSB on P release from lagoon sediments and growth of Chaetomorpha sp.In this work,we examined the influence of OPB inoculation on the concentration of water total P(TP),soluble reactive P(SRP),algal biomass,and tissue P in algae.Furthermore,the variations in P fractions and alkaline phosphatase activity(APA)in sediments pre-and post-experiment were examined.The concentration of TP and SRP in water during the experiment ranged between 0.018-0.621 mg·L-1 and 0.004-0.376 mg·L-1,respectively.The inoculation of OPB significantly promoted P release from lagoon sediments.The amount of OPB inoculation had a significant effect on the TP and SRP concentration in the absence of Chaetomorpha sp.The TP concentration in the high inoculation groups was all higher than that in the low inoculation treatment groups,whereas the SRP showed a significant difference between high and low OPB amount in coexistence with G.stearothermophilus.The APA in sediments of the inoculation group was higher than that of the control group,with a difference value of 0.01-0.81 μmol·g-1·h-1,indicating that OPB could promote P release from sediments by secreting alkaline phosphatase.The concentration of NH4Cl-P and NaOH-P in sediments was lower than the initial value under most treatments.Chaetomorpha sp.grew well with low inoculation of G.stearothermophilus,along with higher tissue P concentration and P enrichment amount than that of other treatments.The inoculation of OPB significantly stimulated P release from sediments and the growth of Chaetomorpha sp.in Swan Lagoon.G.stearothermophilus has a strong ability to secrete APA,which could solubilize P in the sediments of algal-bloom area.Therefore,in terms of the interactions between sediments,PSB and macroalgal blooms,the influence of PSB on the blooms of Chaetomorpha sp.should not be ignored in coastal waters.关键词
有机解磷菌/沉积物/磷释放/碱性磷酸酶/硬毛藻/天鹅湖Key words
organic phosphate-solubilizing bacteria/sediments/phosphorus release/alkaline phosphatase/Chaetomorpha sp./Swan Lagoon分类
资源环境引用本文复制引用
姜晓静,谢嘉慧,马凯,高丽..天鹅湖沉积物中解磷菌的解磷能力及其对硬毛藻生长的影响[J].生态环境学报,2024,33(4):633-644,12.基金项目
山东省自然科学基金项目(ZR2021MD109 （）
ZR2018MD018) （）
