饲料研究2024，Vol.47Issue(7)：49-55,7.DOI:10.13557/j.cnki.issn1002-2813.2024.07.010
灭活植物乳杆菌及其代谢物对克氏原螯虾生长性能和肠道健康的影响
Effects of inactivated Lactobacillus plantarum and its metabolites on growth performance and intestinal health of Procambarus clarkii
摘要
Abstract
The purpose of this study was to investigate the effects of inactivated Lactobacillus plantarum and its metabolite(LPM)on the growth performance and intestinal health of Procambarus clarkii.A total of 162 Procambarus clarkii with an initial weight of(8.16±0.23)g were randomly divided into three groups with three replicates in each group and 18 shrimps in each replicate.Three groups of compound diets containing 0(control group),300(LPM-300 group),600 mg/kg(LPM-600 group)of Lactobacillus plantarum and its metabolite(LPM)were prepared.In the two experimental groups,3％flour was used instead of 3％peanut meal on the basis of the feed formula of the control group,which reduced the protein level of the feed by 1.5％.The trial period was 42 days.The results showed that the weight gain rate,specific growth rate,protein efficiency and protein deposition rate of Procambarus clarkii were significantly higher than those of the control group(P＜0.05),while the feed coefficient was significantly lower than that of the control group when 600 mg/kg LPM was added to the diet(P＜0.05).The contentations of crude protein,crude fat and crude ash of whole shrimp fed with 600 mg/kg LPM were significantly higher than that of the control group(P＜0.05).When 300 and 600 mg/kg LPM was added to the diet,the crude fat content of muscle was significantly higher than that of the control group(P＜0.05).Compared with the control group,the content of serum total protein(TP)increased significantly(P＜0.05),and the activities of aspartate-oxaloacetic transaminase(AST)and alanine-pyruvic transaminase(ALT)decreased when LPM was added to the diet(P＜0.05).The content of serum albumin(ALB)and the activity of alkaline phosphatase(AKP)were significantly increased when 600 mg/kg LPM was added to the diet(P＜0.05).The protease activity of hepatopancreas and intestinal tract was significantly increased by adding 600 mg/kg LPM(P＜0.05).The addition of 600 mg/kg LPM significantly reduced the villus width of the shrimp compared with the control group(P＜0.05).The hepatopancreas cells in the control group and the experimental group were intact without damage or pathological changes.The number of B cells,F cells,and R cells in the LPM-300 group was significantly more than that in the control group.The study indicates that the addition of 600 mg/kg LPM in the feed can improve the intestinal tissue structure of Procambarus clarkii,enhance the non-specific immunity and promote the utilization of feed protein.关键词
克氏原螯虾/灭活植物乳杆菌及其代谢物/生长性能/肠道健康Key words
Procambarus clarkii/inactivated Lactobacillus plantarum and its metabolites/growth performance/intestinal health分类
农业科技引用本文复制引用
石英,庞纪彩,田娟,刘晶晶,高欣,宋晓青..灭活植物乳杆菌及其代谢物对克氏原螯虾生长性能和肠道健康的影响[J].饲料研究,2024,47(7):49-55,7.基金项目
中国水产科学研究院基本科研业务费(项目编号:2022XT01) （项目编号:2022XT01）
山东畜牧兽医职业学院科技发展计划项目(项目编号:2022zrkxzd002、2022370701128088) （项目编号:2022zrkxzd002、2022370701128088）
