深圳大学学报（理工版）2024，Vol.41Issue(3)：358-366,9.DOI:10.3724/SP.J.1249.2024.03358
一株红树林来源放线菌的抗菌活性物质
Antimicrobial compounds of an actinomycete derived from mangrove sediments
摘要
Abstract
Mangrove-derived microorganism is a promising and important resource of novel natural products and lead compounds for drug discovery.This study aimed to find microbial secondary metabolites with novel structures.Based on the bioactive-guided screening of mangrove-derived actinomycetes,Streptomyces sp.GP3T5 was studied owing to its antibacterial,antibiofilm and anti-fungi activities.Three compounds(1-3)were isolated from the fermentation broth by organic solvent extraction,reversed-phase chromatography,Sephadex LH20 gel chromatography,and preparative high-performance liquid chromatography.Their structures were identified as pyrisulfoxin A(compound 1),collismycin A(compound 2),and echinomycin A(compound 3),respectively,via high resolution electrospray ionization mass spectroscopy(HRESIMS),nuclear magnetic resonance(NMR),ultraviolet(UV)spectroscopy.The activity evaluation of compounds 1-3 revealed that all three compounds showed antibacterial and antibiofilm activities.Compound 3 showed potent antibacterial activity against foodborne pathogens with minimum inhibitory concentrations(ρMIC)of 0.06 µg/mL for Listeria monocytogenes and Streptococcus agalactiae.The minimum biofilm inhibitory concentrations(ρMBIC)of compounds 1-3 against Acinetobacter baumannii were 32 µg/mL,16 µg/mL,and 1 µg/mL,respectively.Compounds 2 and 3 showed good antifungal activity against plant pathogenic fungi,with half maximal inhibitory concentrations(IC50)≤10 µg/mL and≤1 µg/mL,respectively.In conclusion,these results on strain GP3T5 derived from mangroves provide valuable insights for further exploration of bioactive compounds from mangrove soil microorganisms.关键词
天然产物/红树林放线菌/链霉菌/次级代谢产物/抗菌/抗生物膜/抗食源性细菌/抗植物致病真菌Key words
natural products/mangrove derived actinomycetes/Streptomyces/secondary metabolites/antibacterial/antibiofilm/anti-foodborne pathogens/anti-plant pathogenic fungi分类
生物科学引用本文复制引用
张骏梁,史蔷,吴昊,张萌,徐颖..一株红树林来源放线菌的抗菌活性物质[J].深圳大学学报（理工版）,2024,41(3):358-366,9.基金项目
National Key Research and Development Project of China(2018YFA0902500) （2018YFA0902500）
Shenzhen Basic Research Foundation(JCYJ20210324093409025) 国家重点研发计划资助项目(2019YFA0902500) （JCYJ20210324093409025）
深圳市基础研究计划资助项目(JCYJ20210324093409025) （JCYJ20210324093409025）
