基于Toll样受体4/髓样分化因子88/核转录因子κB信号通路探讨针刺"蝶腭穴"改善变应性鼻炎大鼠免疫炎性反应的机制OA北大核心CSTPCDMEDLINE

Objective To observe effects of acupuncture at"Die E acupoint"on the protein expression levels of Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear transcription factor κB(NF-κB),transcription factor T-bet(T-bet),and GATA-binding protein-3(GATA-3)in the nasal mucosa and the serum contents of related inflammatory cytokines in rats with allergic rhinitis,so as to explore the mechanism of acupuncture in treating allergic rhinitis.Methods Twenty-four healthy SD rats were randomly divided into blank,model,acupuncture,and sham acupuncture groups,with 6 rats in each group.The rat model of allergic rhinitis was established by using ovalbumin induction.The rats in the acupuncture group received bilateral acupuncture at the"Die E acupoint"with a depth of 15-20 mm,while the rats in the sham acupuncture group received only sham acupuncture(light and shallow acupunture of the skin at the"Die E acupoint").Both interventions were performed once daily for a total of 6 days.Behavioral scores of rats in each group were recorded.Pathological changes of nasal mucosa were observed by H.E.staining.Serum contents of IgE,ovalbumin-specific IgE(OVA-sIgE),interferon(IFN)-γ,interleukin(IL)-4,IL-10 and IL-17 were measured by ELISA and the protein expression levels of T-bet,GATA-3,TLR4,MyD88 and NF-κB p65 in the nasal mucosa were detected by Western blot.Results After modeling,compared with the blank group,rats in the model group showed increased behavioral scores,serum IgE,OVA-sIgE,IL-4,and IL-17 contents,and nasal mucosal GATA-3,TLR4,MyD88,and NF-κB p65 protein expression levels(P<0.05),whereas the contents of serum IFN-γ,IL-10 and the protein expression level of T-bet in the nasal mucosa were decreased(P<0.05).Comparison between the EA and model groups showed that acupuncture intervention can decrease the behavioral scores of rats with allergic rhinitis,the contents of serum IgE,OVA-sIgE,IL-4,IL-17,and the protein expression levels of GATA-3,TLR4,MyD88,and NF-κB p65 in the nasal mucosa(P<0.05),and up-regulate the contents of serum IFN-γ,IL-10,and the nasal mucosal T-bet protein expression level.Sham acupuncture did not have a significant modulating effect on the above indicators.Inflammatory infiltration of nasal mucosa was seen in the model group and sham acupuncture,and the inflammatory reaction was milder in the acupuncture group.Conclusion Acupuncture at"Die E acupoint"can alleviate the symptoms of allergic rhinitis and suppress the inflammation of nasal mucosa in rats,which may be related to inhibiting the TLR4/MyD88/NF-κB signaling and balancing the levels of cytokines of Th1/Th2 and Treg/Th17,and T-bet/GATA-3.