针刺研究2024，Vol.49Issue(5)：463-471,9.DOI:10.13702/j.1000-0607.20221351
电头针调控大脑中动脉栓塞大鼠缺血大脑皮层区CYP27a1/b1、CYP24a及相关炎性细胞因子表达的研究
Electro-scalp acupuncture regulates the expression of CYP27a1/b1,CYP24a and related inflammatory cytokines in ischemic cortex of rats with middle cerebral artery occlusion
摘要
Abstract
Objective To observe the effect of electro-scalp acupuncture(ESA)on the expression of cytochrome P450a1/b1(CYP27a1/b1),cytochrome P45024a(CYP24a),signal transducer and activator of transcription(STAT)4,STAT6,tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-4 in ischemic cerebral cortex of rats with acute ischemic stroke,so as to explore its mechanism in alleviating inflammatory reaction of ischemic stroke.Methods Sixty SD rats were randomly divided into sham-operation,model,vitamin D3 and ESA groups,with 15 rats in each group.The middle cerebral artery occlusion rat model was established with thread ligation according to Zea-Longa's method.Rats in the vitamin D3 group were given 1,25-VitD3 solution(3 ng·100 g-1·d-1)by gavage,once daily for 7 days.Rats in the ESA group were treated at bilateral anterior parietotemporal slash(MS6)with ESA(2 Hz/100 Hz,1 mA),30 min a day for 7 days.Before and after interventions,the neurological deficit score and neurobehavioral score were evaluated.TTC staining was used to detect the volume of cerebral infarction in rats.The positive expressions of CYP24a,CYP27a1 and CYP27b1 in the cerebral cortex of ischemic area were detected by immunofluorescence.The mRNA expressions of STAT4 and STAT6 in the cerebral cortex of ischemic area were detected by quantitative real-time PCR.The protein expression levels of TNF-α,IL-1β and IL-4 in the cerebral cortex of ischemic area were detected by Western blot.Results Compared with the sham-operation group,the neurological deficit score,neurobehavioral score,the percentage of cerebral infarction volume,the positive expression level of CYP24a and mRNA expression level of STAT4,protein expression levels of TNF-α and IL-1β in cerebral cortex were increased(P<0.01),while the positive expression levels of CYP27a1/b1 and STAT6 mRNA,protein expression level of IL-4 were decreased(P<0.01)in the model group.After the treatment and compared with the model group,the neurological deficit score,neurobehavioral score,the percentage of cerebral infarction volume,the positive expression level of CYP24a and mRNA expression level of STAT4,protein expression levels of TNF-α and IL-1β in cerebral cortex were decreased(P<0.01),while the positive expression levels of CYP27a1/b1 and STAT6 mRNA expression level,protein expression level of IL-4 were increased(P<0.01)in the ESA and vitamin D3 groups.Conclusion ESA can alleviate the inflammatory response in ischemic stroke,which maybe related to its function in regulating the balance between CYP27a1/b1 and CYP24a,converting vitamin D into active vitamin D3,inhibiting vitamin D3 degradation,and regulating Th1/Th2 balance.关键词
电头针/缺血性脑卒中/细胞色素P450a1/b1/细胞色素P45024a/炎性反应Key words
Electro-scalp acupuncture/Ischemic stroke/CYP27a1/b1/CYP24a/Inflammation引用本文复制引用
张珊毓,袁博,罗文君,朱玲桂,张延菊,田甜,彭晓云,王金海..电头针调控大脑中动脉栓塞大鼠缺血大脑皮层区CYP27a1/b1、CYP24a及相关炎性细胞因子表达的研究[J].针刺研究,2024,49(5):463-471,9.基金项目
国家自然科学基金项目(No.81960896) （No.81960896）
兰州大学第二医院萃英学子科研培育计划项目(No.CYXZ2019-18) （No.CYXZ2019-18）
