针刺研究2024，Vol.49Issue(5)：472-479,8.DOI:10.13702/j.1000-0607.20230154
培土抑木针法调节CRF/CRFR1通路修复腹泻型肠易激综合征大鼠肠黏膜屏障的机制研究
Mechanisms of Peitu Yimu acupuncture in repairing intestinal mucosal barrier by regulating CRF/CRFR1 pathway in diarrhea-predominant irritable bowel syndrome rats
摘要
Abstract
Objective To investigate the effect of Peitu Yimu(strengthening spleen and soothing liver)acupuncture on intestinal mucosal barrier function and corticotropin-releasing factor(CRF)/CRF receptor 1(CRFR1)pathway in rats with diarrhea-predominant irritable bowel syndrome(IBS-D),so as to explore its underlying mechanism in alleviating IBS-D.Methods Forty female SD rats were randomly divided into blank,model,electroacupuncture(EA),and agonist groups,with 10 rats in each group.Except for the blank group,rats in the other groups were given folium sennae infusion by gavage combined with chronic unpredictable mild stress to establish IBS-D model.Rats in the EA group received acupuncture at"Tianshu"(ST25)and EA at"Zusanli"(ST36)and"Taichong"(LR3)(2 Hz/15 Hz)on one side for 20 min,with the side chosen alternately every other day,for 14 days after modeling.Rats in the agonist group received acupuncture 30 min after intravenous injection of CRFR1 agonist urocortin,with the same manipulation method and time as the EA group.Before and after intervention,visceral pain threshold and stool Bristol scores were measured.Elevated plus maze test and open field test were used to detect anxiety and depression like behavior of rats.ELISA was used to detect the contents of CRF and CRFR1 in rats serum.Immunohistochemistry was used to detect the positive expressions of CRF,CRFR1,zonula occludens protein 1(ZO-1),occlusal protein(Occludin),and closure protein 1(Claudin-1)in colon tissue.Results Compared with the blank group,the visceral pain threshold,open arm time percentage(OT%),total distance of movement in the open field test,and positive expression of ZO-1,Occludin,and Claudin-1 in colon were decreased(P<0.01,P<0.05),while Bristol stool scores,serum CRF and CRFR1 contents,and positive expressions of CRF and CRFR1 in colon were increased(P<0.01)in the model group.After intervention and compared with the model group,the visceral pain threshold,OT%,total distance of movement in the open field test,and positive expressions of ZO-1,Occludin,and Claudin-1 in colon were increased(P<0.05,P<0.01),while Bristol stool scores,serum CRF and CRFR1 contents,and positive expressions of CRF and CRFR1 in colon were decreased(P<0.01)in the EA group;the Bristol stool scores,serum CRF content,and CRF positive expression in colon were significantly decreased in the agonist group(P<0.01).Conclusion Peitu Yimu acupuncture can significantly improve visceral hypersensitivity and anxiety-depression state in IBS-D rats.Its mechanism may be related to the inhibition of CRF/CRFR1 pathway and restoration of intestinal tight junction protein expressions.关键词
腹泻型肠易激综合征/培土抑木针法/电针/肠黏膜屏障/促肾上腺皮质激素释放因子/紧密连接蛋白Key words
Diarrhea-predominant irritable bowel syndrome/Peitu Yimu(soothing the liver and strengthening the spleen)acupuncture/Electroacupuncture/Intestinal mucosal barrier/Corticotropin-releasing factor/Tight junction protein引用本文复制引用
王楷,侯雨君,王路,廖晨希,宋玮,陈颖,周思远..培土抑木针法调节CRF/CRFR1通路修复腹泻型肠易激综合征大鼠肠黏膜屏障的机制研究[J].针刺研究,2024,49(5):472-479,8.基金项目
国家自然科学基金项目(No.82074558) （No.82074558）
