针刺研究2024，Vol.49Issue(5)：480-486,7.DOI:10.13702/j.1000-0607.20230784
电针"四白"和"颧髎"激活小鼠躯体感觉运动皮层的兴奋性神经元
Effect of electroacupuncture stimulation of"Sibai"(ST2)and"Quanliao"(SI18)on excitatory neurons of sensorimotor cortex in mice
摘要
Abstract
Objective To observe the activation state and neuronal types of somatosensory cortex and the primary motor cortex induced by electroacupuncture(EA)stimulation of"Sibai"(ST2)and"Quanliao"(SI18)acupoints in mice.Methods Male C57BL/6J mice were randomly divided into blank control and EA groups,with 6 mice in each group.Rats of the EA group received EA stimulation(2 Hz,0.6 mA)at ST2 and SI18 for 30 minutes.Samples were collected after EA intervention,and immunofluorescence staining was performed to quantify the expression of the c-Fos gene(proportion of c-Fos positive cells)in the somatosensory cortex and primary motor cortex.The co-labelled cells of calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ)and gamma-aminobutyric acid(GABA)in the somatosensory cortex and primary motor cortex were observed and counted by using microscope after immunofluorescence staining.Another 10 mice were used to detect the calcium activity of excitatory neurons in the somatosensory cortex and primary motor cortex by fiber photometry.Results In comparison with the blank control group,the number of c-Fos positive cells,and the proportion of c-Fos and CaMKⅡ co-labelled cells in both the somatosensory cortex and primary motor cortex were significantly increased after EA stimulation(P<0.05).No significant changes were found in the proportion of c-Fos and GABA co-labeled cells in both the somatosensory cortex and primary motor cortex after EA.Results of fiber optic calcium imaging technology showed that the spontaneous calcium activity of excitatory neurons in both somatosensory cortex and primary motor cortex were obviously increased during EA compared with that before EA(P<0.01),and strikingly reduced after cessation of EA compared with that during EA(P<0.05).Conclusion Under physiological conditions,EA of ST2 and SI18 can effectively activate excitatory neurons in the somatosensory cortex and primary motor cortex.关键词
电针/躯体感觉皮层/初级运动皮层/四白/颧髎Key words
Electroacupuncture/Somatosensory cortex/Primary motor cortex/Sibai(ST2)/Quanliao(SI18)引用本文复制引用
张芷晴,申晨薇,陈明权,杜梓霏,陈秋怡,姬昌,张文捷,许能贵,刘健华..电针"四白"和"颧髎"激活小鼠躯体感觉运动皮层的兴奋性神经元[J].针刺研究,2024,49(5):480-486,7.基金项目
国家自然科学基金项目(No.82074544) （No.82074544）
广东省岭南中医药重点研发项目(No.2020B1111100008) （No.2020B1111100008）
国家中医药管理局中医药创新团队及人才支持计划项目(No.ZYYCXTD-C-202004) （No.ZYYCXTD-C-202004）
广东省中医药管理局科研项目(No.20241077) （No.20241077）
广州中医药大学科研创新项目 （）
大学生创新创业训练计划项目(No.202210572018、202310572045) （No.202210572018、202310572045）
