电针对宫腔粘连大鼠子宫内膜M1型巨噬细胞的调控作用OA北大核心CSTPCDMEDLINE

Objective To observe the effect of electroacupuncture(EA)on endometrial fibrosis and M1-type macrophages in rats with intrauterine adhesions(IUA),so as to explore the possible mechanism of EA in the treatment of IUA.Methods Fifteen female SD rats were randomly divided into blank group,model group and EA group,with 5 rats in each group.The IUA rat model was established by double damage method using mechanical scraping combined with lipopolysaccharide infection.Rats in the EA group were treated with acupuncture at"Guanyuan"(CV4),and EA at bilateral"Zusanli"(ST36)and"Sanyinjiao"(SP6)for 20 minutes each time,once a day,for 3 consecutive cycles of estrus.Five rats in each group were sampled during the estrous period,and the endometrial morphology,endometrial thickness and the number of blood vessels and glands were observed after HE staining.The fibrotic area of the uterus was observed after Masson staining.The positive expressions of Runt-related transcription factor(RUNX1),transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),α-smooth muscle actin(α-SMA),collagen type I(Col-Ⅰ),cluster of differentiation 86(CD86),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)in endometrial tissue were detected by immunohistochemistry.Western blot was used to detect relative protein expressions of RUNX1,TGF-β1,α-SMA,CD86,and TNF receptor 2(TNFR2),and real-time fluorescence quantitative PCR was used to detect mRNA expressions of RUNX1,TGF-β1,α-SMA,CD86,and TNF-α in the endometrium.Results During the estrous phase,the endometrial layer in the model group was damaged,with reduced folds,disordered arrangement of epithelial cells,loose fibrous connective tissue,significant narrowing and adhesions in the uterine cavity,interstitial congestion,edema,and a significant infiltration of inflammatory cells with sparse glands.While uterine tissue structure of the EA group was basically intact,resembling a normal uterus,with more newly formed glands and a small amount of inflammatory cell infiltration.In comparison with the blank group,the endometrial thickness,the number of blood vessels,and the number of glands were significantly decreased(P<0.001)in the model group,while the ratio of uterine fibrosis area,the positive expressions of RUNX1,TGF-β1,CTGF,α-SMA,Col-Ⅰ,CD86,IL-1β,and TNF-α,the protein relative expressions of RUNX1,TGF-β1,α-SMA,CD86 and TNFR2,and the mRNA relative expression levels of RUNX1,TGF-β1,α-SMA,CD86 and TNF-α in the endometrium were significantly increased(P<0.001,P<0.01).Compared to the model group,the endometrial thickness,the number of blood vessels,and the number of glands were significantly increased(P<0.01,P<0.05)in the EA group,while the ratio of uterine fibrosis area,the positive expressions of RUNX1,TGF-β1,CTGF,α-SMA,Col-Ⅰ,CD86,IL-1β and TNF-α in the endometrial tissue,the protein expressions of RUNX1,TGF-β1,α-SMA,CD86 and TNFR2,and the mRNA relative expressions of RUNX1,TGF-β1,α-SMA,CD86 and TNF-α in the endometrium were significantly decreased(P<0.001,P<0.01,P<0.05).Conclusion EA can improve endometrial fibrosis in IUA rats,which may be related to its function in decreasing the level of endometrial M1-type macrophages and the secretion of related inflammatory factors.