医学信息2024，Vol.37Issue(9)：101-106,6.DOI:10.3969/j.issn.1006-1959.2024.09.019
氯化钴模拟低氧条件下红景天苷对小鼠成骨细胞的调节作用
Regulatory Effect of Salidroside on Mouse Osteoblasts Under Hypoxic Conditions Simulated by Cobalt Chloride
摘要
Abstract
Objective To study the regulatory effect of salidroside(SAL)on mouse osteoblasts under hypoxic conditions simulated by cobalt chloride(CoCl2).Methods CoCl2 was used to simulate hypoxic conditions(1％02)to establish an in vitro hypoxic model.MTS method was used to study the effect of different concentrations of SAL on the proliferation of MC3T3-E1 cells under hypoxic conditions.The effect of SAL on the differentiation of MC3T3-E1 cells under hypoxic conditions was studied by disodium phenyl phosphate method.Real-time fluorescence quantitative PCR(RT-qPCR),Western blot and ELISA were used to detect the mRNA and protein expression levels of hypoxia-inducible factor-1α(HIF-1α)and downs.Results 0.5 mmol/L CoCl2 was used to simulate the hypoxic(1％02)environment.Hypoxia could significantly inhibit the proliferation and differentiation of MC3T3-E1 cells.After pretreatment with SAL,the proliferation and differentiation of MC3T3-E1 cells were significantly promoted.SAL(10 nmol/L)significantly up-regulated the mRNA expression levels of HIF-1α and VEGF and the protein expression level of HIF-1α,and down-regulated the expression of VEGF protein.Conclusion SAL promotes the proliferation and differentiation of mouse osteoblasts under hypoxia.The specific mechanism needs to be further studied,which may be related to the HIF-1α/VEGF signaling pathway.关键词
红景天苷/成骨细胞/增殖/分化/骨结合/HIF-1α/VEGF通路Key words
Salidroside/Osteoblasts/Proliferation/Differentiation/Osseointegration/HIF-1α/VEGF pathway分类
医药卫生引用本文复制引用
罗婷,祁琳,李杰,薛文舒,田晨晨,曹清文,王越..氯化钴模拟低氧条件下红景天苷对小鼠成骨细胞的调节作用[J].医学信息,2024,37(9):101-106,6.基金项目
1.天津伊特生命科学研发有限公司项目(编号:HX2023-7) （编号:HX2023-7）
2.天津市自然科学基金重点项目(编号:18JCZD-JC33200) （编号:18JCZD-JC33200）
3.天津中医药大学研究生创新基金(编号:ZXY-CXLX202119、ZXYCXLX202213) （编号:ZXY-CXLX202119、ZXYCXLX202213）
