桃苗圃适用除草剂的筛选、混配与安全性评价OA北大核心CSTPCD
Screening,Compounding and Safety Evaluation of Herbicides Suitable for Peach Nursery
[目的]杂草防治是果树生产中的重要环节,论文旨在评价不同除草剂对桃的安全性并筛选适于桃苗圃和生产园利用的除草剂,为桃产业中化学除草的应用提供理论依据.[方法]采用盆栽试验方法,选用毛桃苗以及黑麦草、狗牙根、空心莲子草、白三叶草和黄花苜蓿5种草为试材,对13种除草剂的安全性进行初步筛选.根据各除草剂药害等级结果,选择10%精喹禾灵和60%二氯喹啉酸进行混配.按照精喹禾灵有效剂量25、50、75 mL·hm-2 3个水平,二氯喹啉酸有效剂量150、300、450 g·hm-2 3个水平,共设置形成9个除草剂混配制剂.茎叶喷施处理后7、14、21 d,调查计算杂草抑制率,处理30 d后测定桃苗株高、茎粗、地上和地下部生物量、叶片和根尖细胞电解质渗透率、根系总根长、总根表面积、根体积和根尖数的变化,并基于主成分分析对不同处理的安全性和杂草抑制率进行综合评价.[结果]茎叶喷施处理后,有11种除草剂对桃苗产生药害,桃苗出现不同程度的失绿、萎蔫、枯死等症状,精喹禾灵和二氯喹啉酸对桃苗生长无显著影响.利用精喹禾灵单剂喷施杂草后,处理21 d对禾本科杂草的抑制率为100%,但对阔叶杂草无抑制作用.相反,喷施二氯喹啉酸对禾本科杂草的抑制率为 0,对黄花苜蓿、白三叶草、空心莲子草的抑制率范围为 80%—100%.精喹禾灵与二氯喹啉酸混配后,9 个混配制剂在处理 21 d后的杂草总抑制率可达 90%以上,同时各制剂对桃苗株高、地上和地下部生物量、叶片和根尖细胞电解质渗透率无显著性影响.综合分析显示,精喹禾灵有效剂量对混配制剂的综合评价D值有较大影响,当精喹禾灵有效剂量提升至75 mL·hm-2 时,混配制剂的综合评价D值超过0.563,但D值未出现随二氯喹啉酸有效剂量提升而增大的趋势.[结论]桃苗对多数除草剂敏感,混配是提高精喹禾灵和二氯喹啉酸杂草综合抑制率的有效措施.有效剂量 75 mL·hm-2 精喹禾灵与 300 g·hm-2 二氯喹啉酸混配后,制剂的综合评价D值最高,可在保证桃苗安全的基础上达到最佳除草效果.
[Objective]Weed control is an important step for fruit tree production.To investigate the safety of different herbicides,suitable herbicides for peach nurseries and orchards were screened in peach production,which can provide theoretical basis for the application of chemical herbicides in the peach industry.[Method]To preliminarily screen the safety of 13 herbicides,the peach seedlings combined with Lolium perenne,Cynodon dactylon,Alternanthera philoxeroides,Trifolium repens and Medicago falcata were evaluated in pot.Based on the results of the phytotoxicity index of each herbicide,10%quizalofop-p-ethyl and 60%quinclorac were mixed.According to the effective doses of 25,50,and 75 mL·hm-2 of quizalofop-p-ethyl and 150,300,450 g·hm-2 of quinclorac,a total of 9 herbicide mixtures were established.After 7,14,and 21 d of stem and leaf spraying treatment,the weed inhibition rate was investigated.After 30 d of the treatment,the plant height,stem diameter,aboveground and underground biomass,electrolyte permeability of the leaf and root tip cells,total root length,total root surface area,root volume and root tip number of peach seedlings were also measured.Based on principal component analysis,the safety of different treatments was comprehensively evaluated.[Result]After spraying on the stems and leaves of peach seedlings,11 herbicides caused phytotoxicity in peach seedlings,resulting in varying degrees of symptoms such as chlorosis,wilting,and withering.However,quizalofop-p-ethyl and quinclorac had no significant effect on the growth of peach seedlings.After spraying weeds with a single agent of quizalofop-p-ethyl for 21 d,the inhibition rate of quizalofop-p-ethyl on gramineous weeds was 100%.However,it had no effect on broad-leaved weeds.On the contrary,the inhibition rate of spraying quinclorac on gramineous weeds was 0,and the inhibition rate range of M.falcata,T.repens and A.philoxeroides was 80%to 100%.After being mixed with quizalofop-p-ethyl and quinclorac,the total weed inhibition rate under 9 compound preparations could reach over 90%after 21 d.Meanwhile,each formulation had no significant effect on the height of peach seedlings,aboveground and underground biomass,electrolyte permeability of the leaf and root tip cells.The comprehensive analysis showed that the effective dose of quizalofop-p-ethyl had a significant impact on the comprehensive evaluation D value of the mixed formulation.When the effective dose of quizalofop-p-ethyl increased to 75 mL·hm-2,the comprehensive evaluation D value of the mixed formulation exceeded 0.563,but there was no trend between the increasing D value and the increase of the effective dose of quinclorac.[Conclusion]Peach seedlings are sensitive to most herbicides,and mixing is an effective measure to improve the comprehensive weed inhibition rate of quizalofop-p-ethyl and quinclorac.After mixing an effective dose of 75 mL·hm-2 quizalofop-p-ethyl with 300 g·hm-2 quinclorac,the comprehensive evaluation D value of the formulation is the highest,which can achieve the best weed inhibition rate while ensuring the safety of peach seedlings.
郭磊;黄晨艳;宋宏峰;沈志军;张斌斌;马瑞娟;孙朦;何鑫;俞明亮
江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室,南京 210014
精喹禾灵二氯喹啉酸桃苗除草剂综合评价
quizalofop-p-ethylquincloracpeach seedingherbicidecomprehensive evaluation
《中国农业科学》 2024 (009)
1734-1747 / 14
国家现代农业产业技术体系建设专项(CARS-30)、江苏现代农业(桃)产业技术体系(JATS[2021]425,JATS[2022]426)
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