水稻免疫亲和素基因家族的克隆及对非生物逆境胁迫的响应分析OA北大核心CSTPCD

To explore the function of the immunophilins gene family in rice(Oryza sativa L.japonica Nipponbare)under abiotic stress conditions,the immunophilins gene family members in rice were identified by bioinformatics techniques,followed by cloning,tissue and abiotic stress expression analysis.The results showed that there are 30 OsFKBPs and 29 OsCYPs immunophilins family genes in rice genome.The 30 OsFKBPs were mainly clustered into three large subgroups.The 29 OsCYPs were mainly clustered into four large subgroups.The coding sequences of OsFKBPs and OsCYPs genes were obtained through PCR amplification,and the sequence information was consistent with the predicted in the database.Expression analysis revealed that OsFKBPs and OsCYPs genes exhibited distinct expression patterns in various tissues and developmental stages of rice,with certain genes showing tissue-specific expression.The expression of OsFKBP19 remained consistent across different tissues and developmental stages.OsCYP29 exhibited the highest expression in leaf sheath,while OsCYP57 showed the highest expression in roots.Quantitative PCR results revealed that the expression of the OsCYP37 gene in rice leaves was induced and continuously increased after 4 h of high light intensity stress,but displayed no significant changes under osmotic stress and salt stress.The expression of OsFKBP19 in rice leaves continued to increase after 4 h of high light intensity and osmotic stress,but fluctuated under salt stress.Taken together,OsFKBPs and OsCYPs play important roles in the regulation of high light intensity,osmotic stress and salt stress responses in rice.