Ⅰ群4型禽腺病毒Y17215-1株体外增殖规律及免疫原性研究OA北大核心CSTPCD

To explore the proliferation characteristics and immunogenicity of avian adenovirus type 4 strain Y17215-1,LMH cells were used as a model to study the optimal inoculation dose and harvest time.SPF chickens were immunized via nasal drip of live virus and intramuscular injection of inactivated vaccine.The results showed that the virus titer of the Y17215-1 strain reached the peak of 108.625 TCID50/mL after 48-72 hours of inoculation into LMH cells at 1∶100 dilution.The virus was highly pathogenic to 42-day-old SPF chickens via intramuscular injection and the LD50 was 103.000 TCID50/0.2 mL.21-day-old SPF chickens were intranasally immunized with 107.676 TCID50 of Y17215-1 strain.The neutralizing antibodies were produced in 100％of the chickens at day 7 post immunization and peaked at day 14 days.21-day-old SPF chickens were intramuscularly immunized with the dose of 10 7 676 TCID50/feather of inactivated vaccine prepared from strain Y17215-1.Neutralizing antibodies were detected in 70％of the tested chickens at day 7 post immunization and peaked at day 21.The immunized chickens were challenged with 1000 LD50 of Y17215-1 strain at day 21 post immunization and the protection rates were 100％for chickens immunized with either live or inactivated vaccines.The above results showed that the Y17215-1 strain had good in vitro proliferation and immunogenicity in chickens and might be used as a candidate vaccine strain.