中国动物传染病学报2024，Vol.32Issue(2)：83-88,6.
一株麻鸡传染性法氏囊病毒新型变异株的分离鉴定
Isolation and Identification of a Novel Mutated Strain of Infectious Bursal Disease Virus in Spotted Chicken
王艳 1孙文渊 2朱维 3王峰升 4孟德坤5
作者信息
- 1. 山东省枣庄市农业综合执法支队,枣庄 277800
- 2. 山东省枣庄市畜牧渔业事业发展中心,枣庄 277800
- 3. 山东省滕州市动物疫病预防控制中心,枣庄 277599
- 4. 山东省动物卫生技术中心,济南 250010
- 5. 山东省平度市农业农村局,平度 266700
- 折叠
摘要
Abstract
In recent years,a novel variant of chicken infectious bursal disease virus has been widely prevalent in China,mainly occurring in broiler chickens.It has the characteristics of weak pathogenicity and strong concealment,but it can cause severe atrophy of the bursa of Fabricius.This study collected the atrophic bursa of Fabricius from spotted chickens in Jining area,Shandong Province,to isolate the virus by SPF chicken embryos.The virus was identified through RT-PCR detection and injected to SPF chickens for pathogenicity testing,and then its main antigen gene VP2 was analyzed for evolution.It was found that the strain could kill chicken embryos.Sequencing comparison and evolutionary tree analysis of gene VP2 revealed a close evolutionary relationship with the gene sequences of recent novel variants,with homology ranging from 96.1％to 99.2％.On the 7th day of artificial infection in SPF chickens,there was a significant atrophy of the bursa of Fabricius,and on the 14th day,the atrophy was more pronounced,with a significantly smaller bursa to body ratio than the control group(P＜0.01).The JN22 strain of infectious bursal disease virus isolated in this study belongs to a novel mutant strain,which can cause significant atrophy of the bursal disease in SPF chickens.关键词
鸡传染性法氏囊病毒/新型变异株/麻鸡/法氏囊萎缩Key words
Infectious bursal disease virus in chickens/novel variant strain/spotted chicken/atrophy of the bursa of Fabricius分类
畜牧业引用本文复制引用
王艳,孙文渊,朱维,王峰升,孟德坤..一株麻鸡传染性法氏囊病毒新型变异株的分离鉴定[J].中国动物传染病学报,2024,32(2):83-88,6.
