中国动物传染病学报2024，Vol.32Issue(2)：89-96,8.
CSBV的分离鉴定及其感染幼虫后的应答反应
Isolation and Characterization of Chinese Sacbrood Bee Virusand Experimental Infection in Larvae
摘要
Abstract
The objectives of the present study were to investigate whether the bees on an apiary were infected with Chinese Sacbrood bee virus(CSBV)and to explore the infection features in Apis cerana.The bees suspected to be infected with CSBV were collected from the apiary and the presence of CSBV nucleic acid was examined by RT-PCR.The positive bee samples were collected for virus isolation and purification,electron microscope observation and sequencing.Three-day-old Apis cerana were experimentally infected with the purified and identified CSBV and transcriptome sequencing was performed to analyze the infection features of Apis cerana.As a result,a CSBV-JX strain was isolated and identified through RT-PCR and electron microscope observation.The diameter of CSBV-JX was observed by an electron microscope to be 25-30 nm.The CSBV-JX was amplified and sequenced in segments and the genome sequence was found to be 8781 bp.Phylogenetic analysis showed that the SBV of the Apis cerana(AcSBV)and the SBV of the Apis mellifera(AmSBV)were divided into two branches with solid regional difference.Transcriptome sequencing analysis revealed that 59 genes were up-regulated and 83 were down-regulated in Apis cerana at 2 hours post infection with CSBV-JX and while 68 genes were up-regulated and 86 genes were down-regulated in Apis cerana at 12 hours post infection with CSBV-JX.In summary,this study,the CSBV-JX strain isolated and identified and minor responses to experimental infection in named CSBV-JX.Apis cerana were demonstrated at 2 hours and 12 hours by transcriptomics analysis.The reason for minor infection might be at the early stages of virus multiplication.In early infection stages,the viral loads were low,causing minimal proliferation and replication.However,the differentially expressed genes(DEGs)were highly related to the metabolic pathways in Apis cerana at 2 hours and 12 hours post infection.In conclusion,the results of this study provided a basis for illustrating the molecular mechanism of CSBV infection in Apis cerana.关键词
中蜂囊状幼虫病毒/分离鉴定/系统发育分析/转录组学Key words
Chinese Sacbrood virus/isolation and identification/phylogenetic analysis/transcriptomics分类
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费世港,刘合永,王叶元,冯敏,王子龙,孙京臣..CSBV的分离鉴定及其感染幼虫后的应答反应[J].中国动物传染病学报,2024,32(2):89-96,8.基金项目
广东省地方畜禽品种保护与利用促进项目(2018-143) （2018-143）
国家自然科学基金项目(31860686) （31860686）
