中国动物传染病学报2024，Vol.32Issue(2)：151-159,9.
猪圆环病毒2型和3型检测方法建立及初步应用
Establishment and preliminary application of detection methods for PCV2 and PCV3
摘要
Abstract
Rapid detection and identification of PCV2 and PCV3.In this paper,probe primers were designed with reference to highly conserved fragments of PCV2 and PCV3 in GenBank,and a dual TaqMan fluorescence quantitative detection method was successfully established.The results show that this method has good specificity and can distinguish porcine circovirus type 2 and porcine circovirus type 3 from other porcine disease viruses.The minimum detection limit of PCV2 is 1.00×101 copies/μL,and that of PCV3 is 1.00×100 copies/μL;The repeatability is high,and the coefficient of variation between batches is 0.01％～0.02％and 0.02％～0.05％,respectively.In this study,440 pig samples with clinical symptoms such as reproductive disorder,panting,emaciation and pallor,skin inflammation were collected from large-scale pig farms in Hubei Province,and the established method and commercial kit were used for synchronous detection.The coincidence rate of PCV2 and PCV3 were 97.47％and 96.85％,respectively.The sequencing results showed that PCV2 was subtype 2d,and PVC3 was subtype 3c.The dual TaqMan fluorescence quantitative detection method was characterized by good specificity,sensitivity,rapidity,accuracy and cost saving.It can provide technical support for monitoring and prevention of PCV2 andPCV3.关键词
PCV2/PCV3/双重TaqMan qPCR方法建立/混合感染Key words
PCV2/PCV3/Establishment of dual TaqMan qPCR/mixed infection分类
农业科技引用本文复制引用
李桐,赵润泽,李博天,李春琪,王妍,郭利伟,杨小林,刘国平..猪圆环病毒2型和3型检测方法建立及初步应用[J].中国动物传染病学报,2024,32(2):151-159,9.基金项目
荆州市2022年度自然科学研究专项(202254-07CC) （202254-07CC）
石首市先行县"先进技术集成示范基地建设与定向攻关"揭榜项目(SS202311) （SS202311）
生猪健康养殖省部共建协同创新中心创新平台项目(2019H2209) （2019H2209）
