中国动物传染病学报2024，Vol.32Issue(2)：160-164,5.
卵形巴贝斯虫与中华泰勒虫双重PCR检测方法的建立
Establishment and application of multiplex PCR method for detection of Babesia ovata and Theileria sinensis
摘要
Abstract
In order to establish a fast duplex PCR method to simultaneously detect Babesia ovata and Theileria sinensis.The primers which could specificall amplify the sequences of the AMA1of Babesia ovata,and the 16S rRNA of Theileria sinensis.A multiplex PCR was developed to amplify specific DNA fragment of Babesia ovata and Theileria sinensis with the target Sequence of 986 bp and 500 bp.This method was specific in detecting Babesia ovata and Theileria sinensis.The detection limit was 16 fg/μL for Babesia ovata and Theileria sinensis,respectively.Clinical samples from Shulan in jilin province were detected by the multiplex PCR and the detection rate was 30％for Babesia ovata;16.67％for Theileria sinensis and 10％for mixed infection.It showed that the established multiplex PCR is suitable for quickly clinical detection and molecular epidemiological investigation.关键词
双重PCR/卵形巴贝斯虫/中华泰勒虫/AMA1/MPSPKey words
Multiplex PCR/Babesia ovata/Theileria sinensis/AMA1/MPSP分类
农业科技引用本文复制引用
田万年,迟雪,袁世超,郭小雨..卵形巴贝斯虫与中华泰勒虫双重PCR检测方法的建立[J].中国动物传染病学报,2024,32(2):160-164,5.基金项目
吉林省教育厅科学技术研究项目(JJKH20230454KJ) （JJKH20230454KJ）
