中国动物传染病学报2024，Vol.32Issue(2)：195-200,6.
A型塞内卡病毒VP1蛋白的原核表达及多克隆抗体制备
Prokaryotic Expression of VP1 Protein of Senecavirus A and Preparation of Its Polyclonal Antibodies
摘要
Abstract
Senecavirus A(SVA)is an emerging swine pathogen that can cause vesicular lesions in sows and acute death of piglets.In this study,the VP1 gene of SVA was cloned into the prokaryotic expression vectors pCold-Ⅰ and pCold-TF and then the recombinant plasmids VP1-pCold-Ⅰ and VP1-pCold-TF were verified and transformed into E.coli BL21(DE3)to induce the expression of recombinant protein.The results showed that VP1-pCold-Ⅰ existed in the precipitates(inclusion bodies)and VP1-pCold-TF was a soluble protein.The purified proteins were used to immunize BALB/c mice.The polyclonal antibodies were obtained after immunization four times and detected in Western blotting and indirect immunofluorescence assay(IFA).The prepared polyclonal antibodies had good tiers in Western blot and IFA,which provided a tool for relevant basic and applied research.关键词
A型塞内卡病毒/VP1/原核表达/多克隆抗体Key words
Senecavirus A/VP1/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
秦文珍,于海,单同领,童光志,兰道亮,孔宁,李挺,赵欣,董苏洁,翟焕杰,叶晨倩,叶曼青,童武,郑浩..A型塞内卡病毒VP1蛋白的原核表达及多克隆抗体制备[J].中国动物传染病学报,2024,32(2):195-200,6.基金项目
西南民族大学中央高校基本科研业务费专项资金项目(2020YYXS70) （2020YYXS70）
