中国兽医杂志2024，Vol.60Issue(5)：21-29,9.DOI:10.20157/j.cnki.zgsyzz.2024.05.003
广东省某生猪屠宰场tet(X4)和blaNDM阳性菌的流行特征
Epidemiological Characteristic of tet(X4)and blaNDM Positive Strains in a Swine Slaughterhouse in Guangdong Province
摘要
Abstract
To explore the epidemiological characteristics of tet(X4)and blaNDM positive strains in a swine slaughterhouse in Guangdong Province,94 samples from various sources(55 pig feces,20 slaughterhouse environments,and 19 pig carcasses)were collected.MacConkey agar supplemented with tigecycline and meropenem was used to screen for tigecycline-and meropenem-resistant strains.PCR was employed to detect tet(X4)and blaNDM positive genes,and positive strains were identified.The drug sensitivity of positive strains was determined by the microdilution broth method and agar dilution method.The results showed that the detection rates of tet(X4)and blaNDM positive strains in all samples were 93.62%(88/94)and 51.06%(48/94),respectively.Among them,the detection rate of tet(X4)positive strains was highest in pig feces samples(98.18%,54/55),followed by carcass samples(89.47%,17/19),and environmental samples(85.00%,17/20).The detection rate of blaNDM positive strains was highest in environmental samples(80.00%,16/20),followed by pig feces samples(47.27%,26/55),and carcass samples(31.58%,6/19).The main carriers of the two resistant genes were Escherichia coli,accounting for 95.65%(88/92)and 74.07%(40/54),respectively.Additionally,tet(X4)positive Escherichia fergusonii,Enterobacter cloacae and Morganella morganii,as well as blaNDM positive Klebsiella pneumoniae,Providencia rettgeri and Pseudomonas guariconensis were also detected.The drug sensitivity test results showed that tet(X4)and blaNDM positive Escherichia coli were multidrug resistant,with a resistance rate of 100%to tetracycline and florfenicol for tet(X4)positive strains,and 100%to cefotaxime and ceftazidime for blaNDM positive strains;both types of positive strains were relatively sensitive to colistin.These results indicate that tet(X4)and blaNDM positive strains are severely contaminated in the slaughterhouse,with Escherichia coli being the main species.The contamination of tet(X4)and blaNDM positive strains in the slaughterhouse poses a threat to food safety and public health,highlighting the need to strengthen investigation and long-term monitoring of antibiotic-resistant bacteria contamination in swine slaughterhouses.关键词
tet(X4)/blaNDM/屠宰场/细菌耐药性Key words
tet(X4)/blaNDM/slaughterhouse/drug resistance分类
农业科技引用本文复制引用
林宛楠,高延玲,吕鲁超,蔡钟鹏,卢丽桃,吴敏怡,焦彦翔,岳超,陈家明,冯汇华,韩腾定..广东省某生猪屠宰场tet(X4)和blaNDM阳性菌的流行特征[J].中国兽医杂志,2024,60(5):21-29,9.基金项目
国家重点研发计划项目(2022YFC2303900) （2022YFC2303900）
国家自然科学基金专项项目(32141002) （32141002）
