中国兽医杂志2024，Vol.60Issue(5)：30-38,9.DOI:10.20157/j.cnki.zgsyzz.2024.05.004
2022年青岛地区鸡源和鸭源多黏菌素耐药大肠杆菌流行情况
Epidemiology of Colistin-Resistant Escherichia coli from Chicken and Duck Sources in Qingdao in 2022
摘要
Abstract
To analyze the epidemiological characteristics of colistin-resistant Escherichia coli from chicken and duck sources in Qingdao and provide reference for the clinical application of colistin,samples were collected from chicken and duck feces at slaughterhouses in Qingdao,Shandong Province.Colistin-resistant E.coli were isolated using colistin-containing agar plates.Drug susceptibility,resistance genes,virulence factors,phylogenetic groups,transferability and genetic environment of the mcr-1 gene were analyzed using agar dilution,PCR,conjugation assays,and whole-genome sequencing.The results showed that a total of 26 colistin-resistant Escherichia coli strains were isolated from 133 feces,with an overall isolation rate of 19.55%(26/133),of which the isolation rate from duck sources(24.24%,16/66)was higher than that from chicken sources(14.93%,10/67).Drug susceptibility analysis revealed that all strains were susceptible to tigecycline and meropenem,while resistance rates to other tested drugs were relatively high(42.31%～100%).All isolated strains exhibited multidrug resistance,with chicken-source strains showing more dispersed multidrug resistance compared with duck-source strains,which were more concentrated at 6 and 9 drugs.All 26 isolates carried the colistin resistance gene mcr-1,while the carriage rates of other resistance genes ranged from 7.69%to 96.15%.The tet(B)gene was only carried by chicken-source strains(20.00%),and the carriage rate of the qnrS gene in chicken-source strains(30.00%)was significantly lower than that in duck-source strains(100%).Virulence factor detection revealed that the carriage rates of the tarT,aer,pap,and neuC genes in chicken-source strains(90.00%,70.00%,50.00%,and 30.00%,respectively)were higher than those in duck-source strains(62.50%,12.50%,0%,and 0%,respectively).Phylogenetic analysis showed that the predominant phylogenetic group of colistin-resistant E.coli in Qingdao was group A,accounting for 76.92%.Conjugation assays confirmed the transferability of mcr-1 in some strains.Genetic environment analysis of mcr-1 showed no insertion sequences upstream or downstream of mcr-1.These results indicate that mcr-1 remains the main driver of the prevalence of colistin-resistant E.coli in poultry sources,but there are differences in resistance,resistance genes,and virulence factors between chicken-source and duck-source colistin-resistant strains.Therefore,research and monitoring of the prevalence of mcr-1 in food animal sources should not be ignored.关键词
鸭/鸡/多黏菌素/毒力因子/大肠杆菌/mcr-1Key words
duck/chicken/colistin/virulence factor/Escherichia coli/mcr-1分类
农业科技引用本文复制引用
崔云昊,李道稳,刘志诚,张启迪,刘德俊,宋士凯,衣云鹏,李秋,刘志海,张亚茹..2022年青岛地区鸡源和鸭源多黏菌素耐药大肠杆菌流行情况[J].中国兽医杂志,2024,60(5):30-38,9.基金项目
鲁渝科技协作项目(2022LYXZ030) （2022LYXZ030）
山东省青年科技创新团队项目(2021KJ094) （2021KJ094）
山东省自然科学基金(ZR2020QC199,ZR2020MC188) （ZR2020QC199,ZR2020MC188）
国家自然科学基金(32172911) （32172911）
青岛农业大学高层次人才启动基金(6631121042) （6631121042）
中国博士后科学基金(2021M701105) （2021M701105）
农业农村部智慧养殖重点实验室(部省共建)开放基金(2023-TJAUKLSBF-2107) （部省共建）
