中国兽医科学2024，Vol.54Issue(4)：427-432,6.DOI:10.16656/j.issn.1673-4696.2024.0059
抑制宿主脂肪酸合成关键酶活性影响O型口蹄疫病毒的复制
Effect on FMDV type O replication by inhibition of host fatty acid synthesis key enzymes activities
摘要
Abstract
In order to investigate the effect of fatty-acid biosynthesis in host cells on the replication of foot-and-mouth disease virus type O,the PK-15 cells and the IBRS-2 cells as cell models,respectively,were used to explore the regulation of FMDV replication.The toxic effects of inhibitors on cells were detected by CCK-8 kit to screen the appropriate pretreatment concentration of the in-hibitors.Western-blot and real-time fluorescence quantification(qPCR)were used to detect the regula-tory effects of exogenous addition of C75 and TOFA on FMDV replication.The results showed that,com-pared with the control group,the protein level and transcript level of FMDV were significantly down-regulated after the adding TOFA or C75.In conclusion,inhibition of fatty acid synthesis key en-zymes activities in host cells can reduce efficiency of FMDV replication,which broke a new path for further research on the pathogenic mechanism of FMDV.关键词
口蹄疫病毒/脂肪酸合成关键酶/抑制/影响Key words
foot-and-mouth disease virus/fatty-acid biosynthesis key enzymes/inhibition/effect分类
畜牧业引用本文复制引用
别鑫恬,赵美玉,赵思越,蔺国珍,郑海学,张克山,王明明,杨行,陈国辉,史喜绢,张大俊,赵登率,闫文倩,陈玲玲,何路..抑制宿主脂肪酸合成关键酶活性影响O型口蹄疫病毒的复制[J].中国兽医科学,2024,54(4):427-432,6.基金项目
甘肃省科技重大专项(21ZD3NA001-5,20ZD7NA006-2) （21ZD3NA001-5,20ZD7NA006-2）
国家重点研发计划项目(2021YFD1800100,2021YFD1801300) （2021YFD1800100,2021YFD1801300）
中国农业科学院重大任务项目(CAAS-ZDRW202006-03) （CAAS-ZDRW202006-03）
