北京地区犬猫尿源奇异变形杆菌耐药性及生物被膜形成能力分析OA北大核心

To investigate the drug resistance status of Proteus mirabilis(P.mirabilis)isolated from canine and feline urine in Beijing,and analyze the main resistance mechanisms against β-lactam antibiotics,this study collected 16 canine and 13 feline urine samples meeting bacteriuria criteria from 2018 to 2019,along with 264 canine and 152 feline urine samples with unidentified bacterial isolates.P.mirabilis were isolated and identified,and their resistance to 12 commonly used antimicrobial drugs was tested.Additionally,the presence of β-lactamases and biofilm formation ability in P.mirabilis isolates were analyzed.The results showed that a total of 44 canine(15.7%)and 5 feline(3.0%)isolates were identified as P.mirabilis.The resistance rates of P.mirabilis isolates to azithromycin and doxycycline were 98.0%,while the resistance rates to β-lactam antibiotics ranged from 6.1%to 73.5%,and the multi-drug resistance rate was79.6%.Among the isolates,28 carried extended-spectrumβ-lactamases(ESBLs)with a positivity rate of 57.1%,mainly represented by blaTEM,blaCTX-M-9,and blaOXA-1 genotypes,while 1 isolate carried AmpC β-lactamase(blaCMY-2 genotype).ESBLs-producing strains showed significantly higher resistance rates to ampicillin,chloramphenicol,cefepime,ceftriaxone,and gentamicin compared to non-ESBLs-producing strains(P<0.05).The biofilm formation rate of P.mirabilis isolates was 100%,and there was no significant correlation between the biofilm formation intensity and drug resistance(P>0.05).These findings indicate that P.mirabilis strains isolated from canine and feline urine in Beijing exhibit severe drug resistance,high ESBLs carriage rate,and 100%biofilm formation rate.For clinical treatment,amoxicillin-clavulanic acid with low resistance rates can be considered.