中国兽医杂志2024，Vol.60Issue(5)：46-55,10.DOI:10.20157/j.cnki.zgsyzz.2024.05.006
鸽源ST443肺炎克雷伯菌的分离鉴定和生物学特性分析
Isolation,Identification and Biological Characteristics Analysis of Pigeon Derived Klebsiella pneumoniae Strain ST443
摘要
Abstract
In order to identify the pathogen causing pneumonic disease in racing pigeons at a breeding farm in Jiangsu Province and elucidate its biological characteristics,one strain of Klebsiella pneumoniae was isolated from the lung tissue of pigeons suffering from respiratory diseases.The isolated strain was identified through morphological examination,biochemical tests,PCR amplification,and 16S rRNA gene sequencing.Further analysis of the biological characteristics of the isolated strain was conducted using multilocus sequence typing(MLST),virulence gene detection,capsular serotyping,wzi gene sequencing,pathogenicity analysis,biofilm formation ability determination,antibiotic resistance gene detection,drug sensitivity tests,and traditional Chinese medicine in vitro inhibition assays.The results showed that the isolated strain was a Gram-negative bacillus with blunt-rounded ends under the microscope,identified as Klebsiella pneumoniae through biochemical tests,khe gene PCR amplification,and 16S rRNA gene phylogenetic analysis.MLST typing revealed it belonged to ST443.The strain carried three virulence genes:fimH,uge,and wabG.Capsular serotyping based on wzi gene sequencing identified the strain as serotype K16.Inoculation of healthy chicks with the strain caused varying degrees of lesions in multiple organs,with a median lethal dose(LD50)of 4.68×109 CFU/mL.The strain exhibited moderate biofilm formation ability and carried four antibiotic resistance genes:blaSHV,blaTEM,tetM,and sul1.It was sensitive to ceftriaxone,cefmetazole,amikacin,compound sulfamethoxazole and polymyxin.In vitro,the traditional Chinese medicine realgar demonstrated significant inhibitory effects on the isolated strain.This study provides insights into the prevention and control of pigeon derived Klebsiella pneumoniae infection.关键词
赛鸽/肺炎克雷伯菌/分离鉴定/ST443型/耐药性Key words
racing pigeon/Klebsiella pneumoniae/isolation and identification/ST443 type/drug resistance分类
农业科技引用本文复制引用
陈瑞格,李赟辉,项维,汪厚强,樊大艳,李华明,雷连成,张付贤..鸽源ST443肺炎克雷伯菌的分离鉴定和生物学特性分析[J].中国兽医杂志,2024,60(5):46-55,10.基金项目
国家自然科学基金项目(32273006) （32273006）
