PRRSV经典毒株、高致病毒株、类NADC30毒株及类NADC34毒株鉴别RT-PCR检测方法的建立及应用OA北大核心CSTPCD
Establishment and application of RT-PCR for identification of PRRSV classic strain,highly pathogenic strain,NADC30-like strain and NADC34-like strain
为提高猪繁殖与呼吸综合征病毒(PRRSV)不同流行毒株的快速鉴别诊断能力,本研究对近几年国内外流行的PRRSV不同毒株的Nsp2核苷酸序列进行比对,设计了 1对可用于鉴别4类不同PRRSV毒株的简并引物,建立了鉴别PRRSV经典毒株(C-PRRSV)、高致病毒株(HP-PRRSV)、类NADC30毒株(NADC30-like)及类 NADC34 毒株(NADC34-like)的 RT-PCR 检测方法.结果显示,C-PRRSV、HP-PRRSV、…查看全部>>
In order to improve the ability of the rapid differential diagnosis of different epidem-ic strains of porcine reproductive and respiratory syndrome virus(PRRSV),the Nsp2 nucleotide sequences of the prevalent PRRSV strains in recent years were compared,and the RT-PCR method was established to identify the classical strain(C-PRRSV),the highly pathogenic strain(HP-PRRSV),the NADC30-like strain(NADC30-like)and the NADC34-like strain(NADC34-like).The results show…查看全部>>
蹇艳银;张佳怡;张耕馨;倪民婷;卢淳;师源;王金涛
黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
畜牧业
猪繁殖与呼吸综合征病毒经典毒株高致病毒株类NADC30毒株类NADC34毒株鉴别
porcine reproductive and respiratory syndrome virusclassical strainshighly patho-genic strainsNADC30-like strainsNADC34-like strainsidentification
《中国兽医科学》 2024 (4)
465-471,7
黑龙江省重点研发项目(GA21B004)黑龙江八一农垦大学引进人才科研启动计划项目(XYB201913)
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