中国兽医杂志2024，Vol.60Issue(5)：56-62,7.DOI:10.20157/j.cnki.zgsyzz.2024.05.007
山羊副结核病的诊断和分型鉴定
Diagnosis and Genotyping Identification of Paratuberculosis in Goats
摘要
Abstract
In order to diagnose a case of goats infected with Mycobacterium avium subsp.paratuberculosis(MAP)and identify the genotype of the MAP strain causing infection,so as to provide scientific basis for the diagnosis and control of sheep paratuberculosis,this study conducted a preliminary diagnosis through medical history investigation,post-mortem examination,and histopathological examination.MAP-specific IS900 and IS1311 genes were amplified by polymerase chain reaction(PCR),and strain identification,sequence analysis,and construction of IS900 gene phylogenetic tree were performed after sequencing.The results showed that the diseased goats were extremely emaciated with thin blood.The walls of the jejunum and ileum were thickened,and the mucosal surface formed folds resembling cerebral convolutions.The mesenteric lymph nodes were enlarged and gray-yellow,with gray-white lesions visible on the cut surface.Histopathological examination of intestinal tissues stained with hematoxylin-eosin revealed proliferation of epithelioid cells and lymphocytes in the lamina propria and submucosa of the jejunum and ileum.The cortical area of the mesenteric lymph nodes showed proliferation of epithelioid cells,while the paracortical area showed lymphocyte proliferation.Acid-fast staining revealed acid-fast bacilli clustered within epithelioid cells.PCR amplification of IS900 and IS1311 genes yielded products consistent in size with the target gene fragments.Sequence analysis and construction of IS900 gene phylogenetic tree revealed that the highest homology reference sequences obtained in this study were of type Ⅱ MAP,with homology exceeding 98%.In conclusion,the diseased goats in the goat farm were diagnosed with paratuberculosis,and the infecting strain was identified as type Ⅱ MAP.This study provides reference for the diagnosis and control of paratuberculosis.关键词
副结核病/山羊/诊断/基因分型Key words
paratuberculosis/goat/diagnosis/genotyping分类
农业科技引用本文复制引用
孟维康,赵维宏,李梦园,马薇,丁玉林,吉林台,赵丽,刘永宏..山羊副结核病的诊断和分型鉴定[J].中国兽医杂志,2024,60(5):56-62,7.基金项目
国家乳业技术创新中心项目(2023-JSGG-5) （2023-JSGG-5）
内蒙古自治区自然科学基金项目(2022MS03023) （2022MS03023）
国家自然科学基金项目(32260887) （32260887）
