局解手术学杂志2024，Vol.33Issue(5)：378-382,5.DOI:10.11659/jjssx.03E023069
赖氨酰氧化酶家族在大鼠急性高眼压视网膜中的表达
Expression of lysyl oxidase family in the retina of the acute ocular hypertension rat
摘要
Abstract
Objective To observe the changes of expression of lysyl oxidase family(LOXs)on the retina in acute ocular hypertension(AOH)rats.Methods The SD adult male rats were randomly divided into the control group(CON group)and the AOH group.The rats in the CON group were fed normally without any treatment,and the rats in the AOH group were established AOH model by anterior chamber perfusion.All rats were sacrificed after 2 weeks and retinal tissues were collected.The expression of LOXs[including lysyl oxidase(LOX)and lysyl oxidase-like proteins of LOXL1,LOXL2,LOXL3,LOXL4],and the expression of extracellular matrix(ECM)proteins of Collagen 1/3/4 a1(Col1/3/4 a1),Elastin(Eln),and Fibulin1/4(Fbn 1/4)in the retinal tissues were detected by qRT-PCR.The localiza-tion of LOXs in rat retina was detected by immunohistochemistry.The expression of LOX in retina was detected by Western blot.Results LOXs were expressed to varying degrees in all layers of the rat retina,LOX was mainly expressed in the retinal ganglion cell layer,nerve fiber layer,inner plexiform layer and outer plexiform layer;LOXL1 was mainly expressed in the inner plexiform layer,outer plexiform layer and vascular wall;LOXL2 was mainly expressed in retinal ganglion cell layer,inner plexiform layer and inner nuclear layer;LOXL4 was mainly expressed in the inner plexiform layer and inner nuclear layer;while LOXL3 was only expressed in the vascular wall.Compared with the CON group,the expression of LOX,Col1a1 and Eln in the retina of rats in the AOH group were significantly increased(P<0.05),and there was no significantly significant difference in the expression of LOXL1,LOXL2,LOXL3,LOXL4,Col3a1,Col4a1,Fbn1,Fbn4 mRNAs in the retina of rats between the two groups(P>0.05).Conclusion LOX is highly expressed in the retina of AOH rats,which may be involved in the pathological process of retinal injury caused by high intraocular pressure through ECM remodeling.关键词
赖氨酰氧化酶/细胞外基质/急性高眼压/青光眼/大鼠Key words
lysyl oxidase/extracellular matrix/acute ocular hypertension/glaucoma/rat分类
医药卫生引用本文复制引用
杨曼,邓成敏,谭薇,金昕,朱晓燕..赖氨酰氧化酶家族在大鼠急性高眼压视网膜中的表达[J].局解手术学杂志,2024,33(5):378-382,5.基金项目
贵州省科技厅计划项目(黔科合基础[2020]1Y327) （黔科合基础[2020]1Y327）
