鸡传染性喉气管炎TaqMan探针实时荧光定量PCR检测方法的建立与应用OA北大核心CSTPCD
Establishment and application of TaqMan probe real-time fluorescence quantitative PCR method for detection of infectious laryngotracheitis in chickens
为了建立一种鸡传染性喉气管炎病毒(ILTV)的快速诊断方法,基于ILTV gB基因设计特异性探针和引物,建立一种基于TaqMan探针的实时荧光定量PCR(qPCR)方法.结果显示,ILTV qPCR方法的标准曲线为y=-3.892 9x+44.607,线性相关值(R2)为0.998 8.该方法的最低检测限为1.0× 10 copies/μL,批内与批间重复性检测的变异系数均小于5.64％,且与其他鸡源病原体无交叉反应.临床样本检测结果显示,该qP…查看全部>>
To establish a rapid diagnostic method for chicken infectious laryngotracheitis virus(ILTV),specific probes and primers were designed based on the ILTV gB gene,and a real-time fluorescent quantitative PCR(qPCR)method based on the TaqMan probe was established.The results showed that the standard curve of the ILTV qPCR method was y=-3.892 9x+44.607 with a linear correlation value(R2)of 0.998 8.The lowest detection limit of the method was 1.0× 10 copies/μL,and …查看全部>>
张相霞;王晓梦;张帅
山东省平邑县畜牧发展促进中心,山东临沂 273300山东农业大学动物科技学院,山东泰安 271018山东农业大学动物科技学院,山东泰安 271018
畜牧业
鸡传染性喉气管炎病毒gB基因实时荧光定量PCRTaqMan探针
chicken infectious laryngotracheitis virusgB genereal-time fluorescence quanti-tative PCRTaqMan probe
《中国兽医科学》 2024 (4)
492-497,6
江苏省科技项目(BE2021332,BE2022318)江苏省农业科技自主创新资金项目(CX(23)1004-3)
评论