中国兽医杂志2024，Vol.60Issue(5)：86-90,5.DOI:10.20157/j.cnki.zgsyzz.2024.05.011
沙门菌血清分型方法的比较
Comparison of Salmonella Serotyping Methods
摘要
Abstract
Serotyping is the most basic and crucial epidemiological investigation method for Salmonella,as different serotypes of Salmonella can cause varying clinical symptoms and pose different risks.Rapid and accurate serotyping is of great significance for the prevention and control of livestock and poultry salmonellosis and for public health safety.Therefore,in this study,51 strains of Salmonella(representing 13 serotypes)isolated in China from 1971 to 2020 were serotyped using whole genome sequencing(WGS)and liquid microarray serotyping methods,and the results were compared with the slide agglutination method to analyze the advantages and disadvantages of these three serotyping methods.The results showed that the serotyping results obtained by the slide agglutination method were consistent with the original results for all 51 strains of Salmonella.The WGS serotyping method identified the serotypes of 34 strains,while the liquid microarray serotyping method identified the serotypes of 23 strains,which were consistent with the results of the slide agglutination method.Compared with the other two methods,the WGS serotyping method could identify strains that could not be serotyped by the slide agglutination method and the liquid microarray serotyping method,thus offering advantages in terms of operational time and cost.This study provides valuable insights for the serotyping of Salmonella.关键词
沙门菌/血清分型/玻片凝集法/全基因组测序分型方法/液相芯片分型方法Key words
Salmonella/serotyping/slide agglutination method/whole genome sequencing serotyping method/liquid microarray serotyping method分类
农业科技引用本文复制引用
张璐,徐锦涛,赵琪,张纯萍..沙门菌血清分型方法的比较[J].中国兽医杂志,2024,60(5):86-90,5.基金项目
国家重点研发计划项目(2016YFD0501600) （2016YFD0501600）
