中国兽医杂志2024，Vol.60Issue(5)：91-100,10.DOI:10.20157/j.cnki.zgsyzz.2024.05.012
非洲猪瘟检测方法研究进展
Research Progress of Detection Methods for African Swine Fever
摘要
Abstract
African swine fever(ASF)is an acute,febrile,highly contagious infectious disease in pigs caused by African swine fever virus(ASFV).The outbreak of ASF seriously threatens the safety of the global pig farming industry,causing significant economic losses worldwide.Currently,there is no safe and effective vaccine against ASF,timely and accurate ASF laboratory diagnosis has become an essential tool for epidemic prevention and control.Existing ASF detection methods include serological testing,polymerase chain reaction(PCR),isothermal amplification technology,and clustered regularly interspaced short palindromic repeats and associated system(CRISPR/Cas).However,serological testing,PCR,and CRISPR/Cas require specialized personnel and equipment,while isothermal amplification technology is limited by the type of samples tested and has lower sensitivity.Therefore,existing ASF detection methods cannot simultaneously achieve high sensitivity,rapidity,simplicity,and suitability for on-site testing.In contrast,novel ASF detection methods offer advantages in terms of convenience,efficiency,time and labor savings,and rapid on-site testing.For example,biosensors do not require specialized personnel for operation,artificial intelligence(AI)can reduce labor costs,and they can be used for early epidemic monitoring.Techniques based on magnetic beads do not require complex equipment,microfluidic technology enables high-throughput rapid detection,and magnetic fluid devices can address nucleic acid contamination issues.With the advancement of technology,novel ASF detection methods based on immunochromatographic test strips and various sensors are flourishing.This article summarizes existing ASF detection methods and reviews the development of novel ASF detection methods over the past five years,aiming to provide reference for the scientific prevention and control of ASF.关键词
非洲猪瘟/新型检测方法/生物传感器/人工智能/微流体技术/磁流体装置Key words
African swine fever/novel detection method/biosensor/artificial intelligence/microfluidic technology/magnetofluidic device分类
农业科技引用本文复制引用
陈画菡,朱君海,高飞,简伟俊,黄丽红,亓文宝..非洲猪瘟检测方法研究进展[J].中国兽医杂志,2024,60(5):91-100,10.基金项目
国家自然科学基金项目(31941014) （31941014）
广东省重点研发计划项目(2019B020211003) （2019B020211003）
