中国兽医杂志2024，Vol.60Issue(5)：118-124,7.DOI:10.20157/j.cnki.zgsyzz.2024.05.016
百香果果壳提取物对热应激小鼠的保护作用
Protective Effect of Passion Fruit Shell Extract on Heat Stress Mice
摘要
Abstract
To investigate the protective effects of passion fruit shell extract on heat stress mice,forty healthy clean-grade ICR male mice were randomly divided into four groups:Control group,Heat stress group,Low-dose group,and High-dose group,with 10 mice in each group.All mice were fed with basic pellet feed and had access to normal drinking water.Mice in the Low-dose and High-dose groups were orally administered passion fruit shell extract at doses of 100 and 200 mg/(kg·bw·d),respectively,while mice in the Control group and Heat stress group were orally administered an equivalent amount of saline solution daily.After 14 days of continuous administration,except for the Control group,mice in the remaining three groups were subjected to 2 hours of heat treatment daily for 7 consecutive days,during which the administration continued.At the end of the experiment,the body weight of the mice was measured on an empty stomach,and their weight changes were analyzed.Blood samples were collected to detect the biochemical indicators of serum including alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(Cr),blood urea nitrogen(BUN),and heat shock protein 70(HSP70),as well as the levels of cytokines including tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6),interleukin-2(IL-2),and interferon-gamma(IFN-γ).Liver and kidney tissues were collected from the mice for histopathological examination.The results showed that there was no significant difference in body weight among the four groups of mice after heat stress(P>0.05),but the mice in the Heat stress group lost the most weight.Compared with the Control group,the serum biochemical and cytokine indicators in the Heat stress group were significantly increased(P<0.05).Compared with the Heat stress group,the serum biochemical and cytokine indicators in the Low-dose and High-dose groups of mice were significantly decreased(P<0.05),and the liver and kidney tissue damage in the mice was alleviated,with the High-dose group being better than the Low-dose group.The results indicate that passion fruit shell extract has a certain protective effect on heat stress mice.关键词
百香果果壳/提取物/热应激/血清生化/细胞因子/组织病理学Key words
passion fruit shell/extract/heat stress/serum biochemistry/cytokine/histopathology分类
农业科技引用本文复制引用
周孝琼,张海燕,郭水灵,王萌萌,陆元安,周华林,王华..百香果果壳提取物对热应激小鼠的保护作用[J].中国兽医杂志,2024,60(5):118-124,7.基金项目
福建省科技计划对外合作项目(2021I0044) （2021I0044）
福建省龙岩市科技计划农业类重点项目(2019LYF9020) （2019LYF9020）
