中国兽医科学2024，Vol.54Issue(5)：577-583,7.DOI:10.16656/j.issn.1673-4696.2024.0085
宿主OAS2蛋白抑制非洲猪瘟病毒体外复制的研究
Inhibition of African swine fever virus replication by the host OAS2 protein in vitro
摘要
Abstract
In order to investigate the relationship between host protein 2′,5′-oligoadenylate synthetase 2(OAS2)and the replication of African swine fever virus(ASFV),the present study was conducted to analyze the regulation of OAS2 in the host by ASFV infection by protein immunoblotting(Western-blot)and real-time fluorescence quantitative PCR(RT-qPCR).OAS2 was constructed and synthesized as a eukaryotic expression plasmid and siRNA interfering sequences,and its role was investigated in MA-104 by RT-qPCR and Western-blot.In this study,we analyzed the regulatory effect of ASFV infection on host OAS2 by protein immunoblotting(Western-blot)and real-time fluorescence quantitative PCR(RT-qPCR).We constructed and synthesized the eukaryotic expression plasmid of OAS2 and the siRNA interfering sequences,and we investigated the effects of ectopic overexpression of OAS2 in MA-104 cells and the down-regulation of OAS2 in PAMs on the in vitro replication of ASFV by RT-qPCR and Western-blot.The results showed that the protein and transcript levels of OAS2 were up-regulated after ASFV infection in PAMs cells.Moreover,exogenous overexpression of OAS2 significantly inhibited ASFV replication,and down-regulation of OAS2 expression promoted ASFV replication.In this study,we confirmed that OAS2 could inhibit ASFV replication in vitro by overexpressing and knocking down the host protein OAS2,and the results provide a theoretical basis for further research on host antiviral immunity against ASFV.关键词
非洲猪瘟病毒/OAS2/天然免疫/抑制Key words
African swine fever virus/OAS2/innate immunity/inhibitory分类
农业科技引用本文复制引用
赵思越,赵美玉,李平,郑海学,张克山,郜原,史喜绢,杨行,张大俊,赵登率,陈国辉,陈玲玲,闫文倩,别鑫恬..宿主OAS2蛋白抑制非洲猪瘟病毒体外复制的研究[J].中国兽医科学,2024,54(5):577-583,7.基金项目
国家重点研发计划项目(2021YFD1801300) （2021YFD1801300）
国家生猪产业体系项目(CARS-35) （CARS-35）
中央高校基本科研业务费专项资金资助 （）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2022SDZG02) （2022SDZG02）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-LVRI) （CAAS-ASTIP-2022-LVRI）
中国农业科学院基础科学中心重大任务(CAAS-CSLPDC-202302) （CAAS-CSLPDC-202302）
