中国草食动物科学2024，Vol.44Issue(3)：17-22,6.DOI:10.3969/j.issn.2095-3887.2024.00.023
牛、羊源大肠杆菌对氨基糖苷类抗生素的耐药性及其耐药基因检测
Resistance of Escherichia Coli from Cattle and Sheep to Aminoglycoside Antibiotics and Detection of Its Resistance Genes
摘要
Abstract
In order to study the epidemic characteristics of Escherichia coli from cattle and sheep in Yulin,Shaanxi province,and its resistance to aminoglycoside antibiotics,746 fecal samples from cattle and sheep were collected for bacterial isolation and purification,as well as identification by Escherichia coli specific primer phoA PCR.The identified Escherichia coli were tested for aminoglycoside antibiotic resistance,and five passivating enzyme genes AadA1,AbdB,Aph(3′)-Ⅰ,Aac(6′)-Ⅰb,Aac(3′)-Ⅱ were detected by PCR for homology sequence analysis.The results showed that the total detection rate of Escherichia coli in Yulin area was 42.49％.The drug resistance rates of Escherichia coli from cattle and sheep to six aminoglycoside were 70.7％for streptomycin,64.3％for kanamycin,47.9％for amikacin,45.1％for gentamicin,39.4％for neomycin,and 30.3％for tobramycin,respectively.The multi-drug resistance rate(resistance to 3 or more drugs)was 67.0％.The detection rates of drug resistance genes AadA1 and Aph(3′)-Ⅰ were 79.5％and 83.0％respectively.In summary,the resistance of cattle and sheep Escherichia coli to aminoglycoside antibiotics was relatively strong,and the drug resistance genes were mainly AadA1 and Aph(3′)-Ⅰ.关键词
牛、羊源大肠杆菌/氨基糖苷类抗生素/药敏试验/耐药基因Key words
Escherichia coli from cattle and sheep/aminoglycoside antibiotic/drug sensitivity test/drug resistance gene分类
农业科技引用本文复制引用
白崇生,刘万华,王功帅,陈占莉,高艳,白永平..牛、羊源大肠杆菌对氨基糖苷类抗生素的耐药性及其耐药基因检测[J].中国草食动物科学,2024,44(3):17-22,6.基金项目
榆林市现代农业产业技术体系(动物疫病防控技术体系)支持项目 （动物疫病防控技术体系）
