咀嚼型洁齿骨活性成分体外抑菌试验和临床应用效果评估OA北大核心

In order to assess the improvement of oral health in adult dogs and the in vitro antibacterial effect of the main active ingredients of chewable tooth-cleaning bones,this study qualitatively detected the inhibitory activity of the main active ingredients in chewable tooth-cleaning bones against Streptococcus mutans,Actinomyces viscosus,Lactobacillus acidophilus,Porphyromonas gingivalis,and Fusobacterium nucleatum subsp.nucleatum using the Oxford cup method.Thirty clinically healthy adult dogs were selected and randomly divided into three groups.After ultrasonic teeth cleaning,the Control group was fed normally,while Test groups 1 and 2 were fed with chewable tooth-cleaning bones of different brands.Every 30 days,the same veterinarian evaluated the dental plaque,calculus,and gingivitis of the test dogs and measured their breath odor.The results showed that the active ingredients in chewable tooth-cleaning bones,such as tea polyphenol,magnolol,Eucommia ulmoides extract,honeysuckle extract,lysozyme,and preservative complex enzyme,had relatively good inhibitory effects on the growth of five common oral pathogens.After 90 days of teeth cleaning,the dental plaque and tartar scores of Test groups 1 and 2 were significantly lower than those of the Control group(P<0.05).After30 and90 days of teeth cleaning,the gingivitis scores of Test group 2 were significantly lower than those of the Control group(P<0.05).Compared with the Control group,feeding chewable tooth-cleaning bones after teeth cleaning significantly improved the breath odor of the dogs.The breath odor values of Test group 2 were lower than those of Test group 1,but the difference was not significant(P>0.05).These results indicate that chewable tooth-cleaning bones can alleviate the oral health of dogs to some extent,and tooth-cleaning bones with added active ingredients have relatively good effects.