中国兽医科学2024，Vol.54Issue(5)：658-665,8.DOI:10.16656/j.issn.1673-4696.2024.0080
新疆部分地区马粪源大肠杆菌的分离鉴定与耐药性及毒力基因检测
Isolation,identification,drug resistance and virulence genetic testing of Escherichia coli from equine faeces in some areas of Xinjiang
摘要
Abstract
To investigate the presence of Escherichia coli in specific areas of Xinjiang,56 stool samples were collected from horse farms,which were confirmed to be connected with E.coli.The drug-sensitive paper method and the microporous crystal violet plate method were used to determine the characteristics of the isolates,and their virulence and resistance genes were detected.The results revealed that 41 out of 56 samples yielded E.coli strains,indicating an isolation rate of 73.2%(41/56).The isolates exhibited varying degrees of resistance to rifampicin(97.6%),cefathiophene(46.3%),cotrimoxazole(29.3%),tetracycline(24.4%)and chloramphenicol(19.5%).Furthermore,7 isolates demonstrated moderate biofilm forming ability(17.1%,7/41),32 isolates had weak biofilm forming ability(78.0%,32/41),and 2 isolates had no biofilm forming ability(4.9%,2/41).Among the 16 examined virulence genes,5 were detected in the isolates,including fimC(87.8%,36/41),fyuA(58.5%,24/41),irp2(41.5%,17/41),sepA(36.6%,15/41)and hlyF(22.0%,9/41).Additionally,11 drug resistance genes were analyzed,and 8 drug-resistant genes parC,gyrA,and gyrB were identified in all isolates(100.0%,41/41).Other detected drug resistance genes included sul2(31.7%,13/41),floR(22.0%,9/41),blaTEM(22.0%,9/41),blaCTX(12.2%,5/41)and aadA(2.4%,1/41).This study provides valuable experimental data on the levels of antibiotic resistance,biofilm-forming ability,and the presence of virulence and drug resistance genes in equine E.coli from various regions of Xinjiang.These findings contribute to the understanding and potential clinical treatment and control of equine colibacillosis in Xinjiang.关键词
大肠杆菌/生物被膜形成/毒力基因/耐药基因/马/新疆Key words
Escherichia coli/biofilm formation/virulence gene/drug resistance gene/horse/Xin-jiang分类
农业科技引用本文复制引用
李静,刘鏊,陀海欣,于月通,马志远,王甜,吴静,张秀萍,齐萌..新疆部分地区马粪源大肠杆菌的分离鉴定与耐药性及毒力基因检测[J].中国兽医科学,2024,54(5):658-665,8.基金项目
新疆生产建设兵团重点领域科技攻关计划项目(2020AB025) （2020AB025）
塔里木大学校长基金(博士)胡杨英才项目(TDZKB-S202307) （博士）
